NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

Mammalian Expression Vectors
Enhanced recombinant protein expression in mammalian cells, superior genetic immunization

NTC Products and Services

VECTORS
XMammalian Vectors
XAntibiotic-Free Vecotrs
XRig-I Activating Vectors
XpDNAVACCUltra Vector
XLinear Vectors
XRecombineering Vectors
XAutolytic E. coli Cell Lines
XGene Sllencing (transient)
XGene Sllencing (retroviral, stable)

XAnti-Silencing Elements

CELL LINES
XAutolytic E. coli Cell Lines

DNA VACCINES
X
Prescion Cloning

RECOMBINANT PROTEINS
X
pVEX™
X
Fluorescent Proteins

PLASMIDS
X
Small Scale Plasmid DNA
X
Small Scale C of A (PDF)
X
Large Scale Plasmid DNA
X
Large Scale C of A (PDF)
X
Plasmid DNA Order Form (PDF)
X
E. coli Cell paste
X
Plasmid Detox Vector, NEW!
XX
DNA FERMENTATION PROCESS
X
HyperGRO™ Process
X
HyperGRO™ Licensing
X
HyperGRO™ Pricing

PROCESS DEVELOPMENT
X
Process Development Basics
X
Process Licensing


HYPERTAQ™ DNA POLYMERASE




Enhanced recombinant protein expression in mammalian cells (gene therapy)
Superior genetic immunization (DNA vaccines)
DNA Vaccination
Therapeutic Vector
dcm(-) or dcm(+)
Native Expression
Secreted (TPA tag)
Minimal Backbone
Reporter (EGFP)
Regulatory Compliance
Simultaneous Cloning
20% Production Savings


pDNAVACCUltra™ DNA Vaccine Vectors
A typical advanced NTC vector, NTC8685-EGFP, with the following features: markerless (sucrose selection), enhanced transcription (SV40/CMV), enhanced translation (CMV/HTLV-1-R), enhanced translation (VA1), enhanced plasmid production (PAS-BH)

Plus, choose from the following special advantages listed in the Flowchart below
and browse through each vector's User Manual for more information.

XXVector Selection Flowchart

Selection
Transgene Targeting
HTLV-I R transient expression enhancer
VA1 transient expression enhancer
SV40-PAS-BH increased plasmid yield  backbone
RIG-I activating backbone
Vector
Kan Secreted; GPI anchored; Proteosome; Endosome; or  Native
No
No
No
No
pDNAVACCUltra™
Sucrose
Secreted; or Native
Yes
No
No
No
NTC8382
NTC8385
Sucrose Secreted; or Native
Yes
Yes
No
No
NTC8382-VA1
NTC8385-VA1
Kan Secreted; or Native
Yes
No
*Yes
No
NTC7482
NTC7485
Kan Secreted; or Native
Yes
Yes
*Yes
Yes
NTC7482-eRNA41H
NTC7485-eRNA41H
Sucrose Secreted; or Native
Yes
No
*Yes
No
NTC8482
NTC8485
Sucrose Secreted; or Native
Yes
Yes
*Yes
Yes
NTC8482-eRNA41H
NTC8485-eRNA41H
Sucrose Secreted; or Native
Yes
Yes
*Yes
No
NTC8682
NTC8685

*Save 20% off large scale production cost with NTC’s high-yield, SV40-PAS-BH vectors!
(for a limited time)

XXFigures 1-2


Figure 1: EGFP transgene expression (fluorescence) in human HEK293 cell line after transfection
of dcm+ or dcm- NTC8685, gWIZ and pVAX1 CMV promoter plasmid vectors.

EGFP transgene expression (fluorescence) in human HEK293 cell line after transfection of dcm+ or dcm- NTC8685, gWIZ and pVAX1 CMV promoter plasmid vectors.


Figure 2: NTC8385-VA1 expression compared to gWIZ-EGFP and pVAX1-EGFP in HEK293 cells.

Figure 2: NTC8385-VA1 expression compared to gWIZ-EGFP and pVAX1-EGFP in HEK293 cells.

XXVector List

Contact NTC, Toll free: 1-(888) WIZ-BANG

Plasmids, Recombinant Proteins, DNA Vaccines, Viral Vectors, Therapeutic Genes, Fermentation Technology for Gene Therapy and Biotechnology
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including Databases and Sequences Analysis Tool
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