1.) contamination from organism (either celld debris of foriegn DNA).
2.) if doing phenol-chloroform extraction, wrong ratio could be used and hence no formation of layers.
3.) salt concentrations/alcohol concentrations wrong and hence no DNA or no DNA precipitation.
good luck.
1.) contamination from organism (either celld debris of foriegn DNA).
2.) if doing phenol-chloroform extraction, wrong ratio could be used and hence no formation of layers.
3.) salt concentrations/alcohol concentrations wrong and hence no DNA or no DNA precipitation.
good luck.