Are there any disadvantages of using adenoviruses instead of retroviruses as vectors in gene therapy

Technorati Tags: gene therapy, retroviruses, vectors

1.What are the two types of vectors used in recombinant DNA technology, and give short examples of each please.
2. How are restriction enzymes important tools in genetic engineering?
3. Why is it essential that both the plasmid and the donor DNA be exposed to the same restriction enzyme?
4. What must researchers know before they begin the process of Genetic engineering?
5. Why does producing a single bacterium through genetic recombinant enable researchers to produce large numbers of bacteria with the recombined plasmid?
6. What would be the goal of a researcher who inserted the gene for insulin onto bacteria?
Thanks! I was out of school for three days and have 7 biology worksheets now!

Technorati Tags: bacteria, bacterium, biology, donor dna, important tools, insulin, large numbers, plasmid, process of genetic engineering, recombinant dna technology, researcher, restriction enzyme, restriction enzymes, vectors

Adding Two or More Vectors

Addition of Vectors

Definition of Addition of Vectors

Adding two or more vectors to form a single resultant vector is known as Addition of Vectors.

More about Addition of Vectors

If two vectors have the same direction, then the sum of these two vectors is equal to the sum of their magnitudes, in the same direction.

If the two vectors are in opposite directions, then the resultant of the vectors is the difference of the magnitude of the two vectors and is in the direction of the greater vector.

Examples of Addition of Vectors

To find the sum of the vectors of  and, they are placed tail to tail to form two adjacent sides of a parallelogram and the diagonal  gives the sum of the vectors  and. This is also called as ‘parallelogram rule of vector addition’.

If the vector is represented in Cartesian coordinate, then the sum of the vectors is found by adding the vector components.

The sum of the vectors and is

=

Solved Example on Addition of Vectors

Let and .  Which of the following is the component form of the vector u + v?

Choices:

A.

B.

C.

D.

Correct Answer: A

Solution:

Step 1: The two vectors are and .

Step 2:u + v =

Step 3: =

Step 4: =

Related Terms for Addition of Vectors

Direction

Magnitude

Parallelogram Rule

Vectors

Additional Links for Addition of Vectors

Click here for samples

Back to Mathematics Dictionary

Technorati Tags: Adding, More, vectors

Add the ffg. vectors:
50g S, 50 degrees E; 50g S, 90 degrees; 100g S, 60 degrees W; and 75g, 0 degrees W. Find the resultant vector with solution.

Technorati Tags: resultant vector, vectors

20. Plant cells that have had the cell walls removed are called ______.
a. plasmids
b. probes
c. vectors
d. protoplasts
e. transgenic

21. A retrovirus has ______ genes.
a. RNA
b. DNA
c. bacterial
d. defective
e. no

22. A retrovirus used in gene therapy works by ______.
a. replacing the patient’s genes with normal viral genes
b. replacing the patient’s DNA with viral RNA
c. using reverse transcription to produce normal human DNA
d. using reverse transcription to produce normal human RNA
e. using polymerase chain reaction to produce normal human DNA

Technorati Tags: cell walls, chain reaction, dna c, gene therapy works, human dna, plant cells, Plasmids, polymerase chain reaction, probes, protoplasts, retrovirus, reverse transcription, transgenic, vectors, viral genes, viral rna

For example: vectors with CMV-IE or EF1 alpha promoters. Construct size ranges between 4-12 kb

Technorati Tags: cmv ie, ef1, promoters, size ranges, vectors

Hey everyone!

At the moment, I am working on a project, but I cannot seem to understand something. In order to create a organism with a foreign DNA sequence, there is a method that must be followed. Correct? Example, if I wanted to grow antlers on a rabbit, I’d take the gene for antlers from a deer and place it into the rabbit’s DNA. Is this the correct method?

1) PCR
2) RFLP
3) Edman Method
4) Blotting
5) DNA Extraction
6) Plasmids
7) Vectors

If this is the correct sequence, can you please explain why each step is present? I understand that the PCR step is their to make copies of the DNA, but what about the RFLP and the others?

Thanks so much!

Technorati Tags: correct sequence, deer, dna extraction, dna sequence, hey everyone, organism, pcr, rabbit, rflp, vectors

Suppose you want to clone a gene from the fungus Neurospora into a bacterial plasmid. The plasmid that you want to use contains genes for resistance to the antibiotics kanamycin (kanr) and tetracycline (tetr).
To prepare for cloning, you treat the plasmid and the gene from Neurospora with the restriction enzyme BglII, which cuts once within the kanr gene in the plasmid. BglII cuts on each site of the Neurospora gene. You then ligate the Neurospora gene into the BglII site in the plasmid and transform E. coli cells.
a.
What single antibiotic would you put in the growth medium to ensure that each colony contains a plasmid?
b.
What antibiotic‐resistance phenotypes would be found among the resulting E. coli colonies?
c.
What will be the antibiotic‐resistance phenotype of the bacterial colonies that contain recombinant vectors (i.e. contain the Neurospora gene).

Technorati Tags: antibiotic resistance, antibiotics, bacterial colonies, bacterial plasmid, cells, e coli, fungus, Genes, growth medium, kanr, neurospora, phenotype, restriction enzyme, tetr, tetracycline, vectors

Which one of the following vectors can be used to clone the longest segments of genomic DNA?

A.Bacteriophages.
B.Bacterial artificial chromosomes.
C.Yeast artificial chromosomes.
D.Plasmids.
E.Retroviruses.

Technorati Tags: bacterial artificial chromosomes, bacteriophages, genomic dna, segments, vectors, yeast

if i have a matrix with more columns than rows, then what would be the span of the vectors after i apply row-echelon form?

thank you

Technorati Tags: echelon, matrix, row echelon form, span, vectors