I have learned of a method by which scientists isolated an E.coli origin of replication and determined a minimal ori. It is done by making a genomic library by introducing different fragments of an E.coli into one E.coli and then seeing which one reproduces which would mean that it contained the origin. Then an enzyme was introduced, that chewed away at the gene from either size until it was determined what the origin size was

I need to modify it appropriately so that you can isolate and define a minimal E.coli promoter.

I am specifically interested in knowing about the selection scheme, modifications of the vector platform if necessary, any reporter gene assays etc – so please highlight these in your approach and provide me with a rationale for any alterations from the initial method. Thank you!
instead of lacz can you uase a temperature sensitive mutant?

Technorati Tags: alterations, e coli, fragments, genomic library, initial method, ori, origin of replication, promoter, rationale, reporter gene, scientists, selection scheme, vector

ok i know i should do all my hw myself but i dont know where to even start!!! please help!!!!

1st one

your teamat the national institutes of health is working on a gene therapy treatment for the delta F508 mutation in a cystic fibrosis transmembrane conductance regulator gene. using an adeno-associated virus as the vector, describe the procedures your group will employ to prepare for and successfully deliver a healthy gene to cells of the affected tissue

k here is the next one

your hot shot biotech team attempts to straighten out a potential problem. some lab technicians might have accidentally mixed samples of DNA from several sources. Using the Southern Blot Technique, describe how you can identify the DNA fragments in the mixture that carry the PAH gene (a mutation in this gene, located on chromosome 12, can cause phenylketonuria)

im pretty sure for the second one you would compare a straight sample of PAH to the DNA samples

Technorati Tags: adeno associated virus, cells, chromosome, cystic fibrosis, cystic fibrosis transmembrane conductance regulator, delta, dna fragments, dna samples, gene therapy treatment, hw, institutes of health, lab technicians, mixture, mutation, national institutes of health, pah, regulator gene, southern blot technique, vector

type of vector they used? Did they use a plasmid, gene gun, virus?

Technorati Tags: gene gun, vector, virus

10. Which one of the following statements about using reverse genetic techniques to produce disease models
in knockout mice is INCORRECT?
(A) The sequence of the gene must be known before the gene can be knocked out.
(B) The targeting vector contains a disrupted copy of the gene where all or part of that gene has been
replaced by a selectable marker.
(C) The targeting vector is transformed into embryonic stem (ES) cells, where it will recombine with the
ES cell DNA.
(D) The knockout mice are missing one good copy of the gene in all cells.

Technorati Tags: cells, disease models, dna, embryonic stem, genetic techniques, knockout mice, marker, selectable marker, vector

a. Vector
b. Reverse Transcriptase
c. Trasnport molecule
d. Genetic probe
e. PCR device

Technorati Tags: molecule, pcr, reverse transcriptase, vector

I am trying to solve question 3 from the wikibook here: http://en.wikibooks.org/wiki/Linear_Algebra/General_%3D_Particular_%2B_Homogeneous .
The solution says simply plug in, however I do not know how to do this.
The homogeneous vector solution I get is:
(3)
(5)
(-7)
(1)

If I insert each of the particular solutions in turn, for the definition of ‘w’ in part c I would get:
w = -1 + w.

This cant be correct – what am I doing wrong?

Thanks.
I am trying to solve question 3 from the wikibook here: http://en.wikibooks.org/wiki/Linear_Algebra/General_%3D_Particular_%2B_Homogeneous .
The solution says simply plug in, however I do not know how to do this.
The homogeneous vector solution I get is:
<3, 5, -7, 1>

If I insert each of the particular solutions in turn, for part 3 I would get:
general: <3, 5, -7, 1>
particular: <-1, -4, 8, -1>

Thus if I take each of these equations in order I would have:
x = 3w – 1
y = 5w -7
z = -7w + 8
w = w – 1

The last statement cant be correct – what am I doing wrong?

Thanks.

Technorati Tags: 5w, 7w, linear algebra, lt 1, vector, wikibook

Beltronics Vector 955: Get the Benefits

Beltronics Vector 955 is a great unit you must have if you are looking for the proper radar detector for your needs. This device is said as the best option of detector because it is easy to use and it is seen as the basic of the detector one for your needs. It is available in the market and you could purchase this unit for 5. And then, you will be able to take benefits from it so your money will be valuable for the stuff you have purchased. In this case, purchasing this product will be a great thing for you so it is important for you to have this kind of product.

Although it is a great option for you, this unit only gives shorter warning of radar. This product is provided with some good features that will make you amazed because it will be useful for you in knowing the speed you have already taken when you are driving in. The product is affordable but the features given are almost equal with the expensive one, so taking this option will give you more benefits. This product can be said as the best solution if you would like to avoid getting more traffic tickets that will cost you with some money.

This collection could be said as the right product you need to perform because it seldom makes false alarms so it will be helpful for you. In purchasing the product for your needs, you must be sure that you make the proper choice for you so you will not feel sorry if you do something wrong in the purchasing process. That product is able to give plenty of warning so it will warn you if you take the excessive speed. Thus, it is advised to take this unit for using at your motor or car.

Technorati Tags: Beltronics, Benefits, vector

Vector Informatics releases Skype Contact Explorer internet marketing tool!

Vector Informatics, Inc. – a company that provides internet, technology, and marketing services to car dealerships, has released Skype Contact Explorer free for registered users.

May 27, 2010 (MMD Newswire) — Skype Contact Explorer is a companion for Skype that installs in Windows 7, XP, Server x. The companion is a powerful communication tool allowing you to maximize your Skype account when using as your primary means of communication. Send messages faster, to more users. Tap the potential of the Skype user base.

FEATURES
• Contact multiple users at once
• Add multiple users to your contacts at once
• Message all of your contacts at once
• Search the Skype database using keyword files
• Search for Skype users based on your business
• Save your searches
• Build a rolodex of Skype usernames
• Send messages to targeted groups

Skype is changing the way we communicate over the internet. Companies are turning to Skype more and more for a communications solution instead of VOIP or traditional landlines. Communicating with this growing user base is easiest done on their native platform. Users who are contacted over Skype are more responsive than being called on a telephone.

Skype Contact Explorer is freeware with limitations – to remove the limitations and pay for an unlimited amount of messages sent, SCE is lifetime unlimited license.

To get more information about the Skype Contact Explorer, please visit: Skype Contact Explorer

Skype Contact Explorer is a production of Vector Informatics Dealer SEO and Internet Marketing

For more information, please visit following link: Vector Informatics Dealer SEO and Internet Marketing

Technorati Tags: Contact, Explorer, Informatics, internet, Marketing, Releases, Skype, Tool, vector

Vector Electrolysis Hair Removal System

Vector Electrolysis System started in 1998 to serve the apparent absence of any true permanent hair removal technologies for private use. It is designed to offer a new solution, allowing consumers to permanently remove unwanted hair in their homes. Consumers always complain of getting hard tweezing, waxing depilation, electrolysis and even laser. All of these procedures are time consuming, painful, messy, frustrating and expensive.

Vector System is a type of product that we’re using within ourselves and share it to our friends and family members. It’s better to get rid of unwanted hairs and worry free about it anymore.

Description

Enables permanent removal of hair.
Guarantees professional results without the use of needles.
Effective on all types of skin.
Gets rid of the thickest hair of any color.
Same results as professional electrolysis.
Permanent effects and the lesser cost than professional electrolysis.
Saves time, as tweezed hair without electrolysis will grow back and must be repeatedly removed.
Tired of using gadgets, lotions, or razors to remove hair, none are completely successful and a waste of your time and money.
The effects are permanent, and you have the machine forever.
Certified by the American FDA.
Comparing the Vector from other devices which use broadcasting frequency and batteries, this product uses electric power. It gets excellent results without the use of a needle. Procedure is not painful and simple to use.

Contents of Kit:
Vector Machine
8 electrode Latex Patch
Electrically conductive gel
Galvanic tweezer
Cable.
Further Information
Hair removal is irreversible.
You should read the instructions completely before beginning treatment.
Do not use in the shower, bath or near water.
Cautiously choose places to remove hair.
Using force can cause scalding.
Keep Vector stored away from children.
Disconnect after use and store in a secure place.

Visit NaturalLabs.co.uk for  Vector Electrolysis System and hair loss treatment.

Technorati Tags: Electrolysis, Hair, Removal, System, vector

Blow your Pictures; Convert Jpg files to Vector

A Vector Image is a representation of an image as an array of pixels, the images are often photographic.

Computer monitors are made of up tiny rectangular cells called pixels and images are constructed from these tiny cells. The size and distance of the cell from each other determines the quality of the images produced – the smaller and closer, the better. This in essence increases the size of files needed to store the data.

When images are magnified and the number of pixels is kept the same, the number of each pixel grows therefore, causing the image to become pixilated when viewed with the eyes. This means that a small picture becomes grainy when it is zoomed out or the resolution is increased beyond a certain threshold.

Designers convert jpg to vector to eliminate this problem.  Vector graphics stores the shapes, line and color that make up an image as a mathematical formula. Hence, when enlarging the image, the vector program recreates the image using this formula thereby creating the best possible screen image with the given screen resolution.

This means that since the vector program recreates the image using mathematical formulae, it can scale the image to any size as long as the screen resolution permits it. Printing such images to paper gives a much sharper and clearer image with higher resolution than what shows on the screen.

The software to convert jpg files to vector is usually very expensive. The much less expensive ones have limited color reproduction capacity.

To convert jpg to vector with unlimited color capacity, open-source software might be the solution. Follow the steps carefully.

1.  Browse for an open source vector imaging software called Inkscape.

2.  Click the download link and run the program to install it on your computer.

3.  After installation, click ‘Open Program’. Not much advanced knowledge is needed to use this program as it is very user-friendly.

4.  Select ‘File’ from the drop-down menu bar at the top and, select ‘Import’. Choose the Jpg file you need to convert and proceed.

5.  From the drop-down menu bar, select ‘Path’ and then ‘Trace Bitmap’. This action opens another window to adjust settings and there is a preview of the changes on the right.

6.  Select the ‘Colours’ option and increase the number of ‘Scans’ several times

7.  Click on the ‘Update’ menu.  Increasing the number of scans increases the resolution, the file size and the speed at which the document will render. Play around with the settings to suit your image requirements and update it regularly.

8.  Save the image as an .eps file and your jpg image is now vector!

Technorati Tags: Blow, Convert, files, Pictures, vector