A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

I have some serious questions I need help with, if you can answer any of them it’d be sweet THANKS

1. Golden rice is golden because it is rich in ______.

a. vitamin A
b. vitamin C
c. beta-carotene
d. chromium picolinate
e. protein

2. Gel electrophoresis separates DNA fragments based on differences in their _____.

a. A:C ratio
b. G:T ratio
c. G:C ratio
d. length
e. polar charge

3. Cutting DNA with a particular restriction enzyme produces _____ that can be separated by gel electrophoresis.

a. restriction fragments
b. enzymes
c. recombinant DNA
d. a genomic library
e. plasmids

4. Which of these can act as a vector to introduce new genes into a bacterial cell?

a. humulin
b. RFLP
c. PCR
d. clones
e. plasmids

I’m finishing up a packet for biology but I can’t get these final questions:

1. To produce transgenic bacter that make insulin, which of the following steps did scientists have to take first?

A: Insert the human insulin gene into a plasmid.
B: Extract the insulin from the bacterial culture
C: Use a restriction enzyme to cut the insulin gene from human DNA.
D: Transform bacteria with the recombinant plasmid.

2. Why is Dolly (the sheep clone by (Ian Wilmut) a clone?

A: The source of her DNA was a single body cell.
B: The DNA molecules in all her cells are identical.
C: She was produced using the DNA from an adult’s egg cell.
D: She is genetically identical to her offspring.

3. Human females produce egg cells that have

A: one X chromosome only.
B: two X chromosomes
C: One X or one Y chromosome
D: One X and one Y chromosome

4.A person who has PKU

A: Inherited the recessive allele for the trait from one parent.
B: Inherited the recessive allele for the trait from both parents.
C: is heterozygous for the trait.
D: Will not pass the allele for the trait to his or her offspring.

5. If a man with blood type A and a woman with blood type B produce an offspring, what might be the offspring’s blood type?

A: AB or O
B: A,B, or O
C: A, B, AB, O
D: AB only

6. The sequencing of human chromosomes 21 and 22 showed that

A: Some regions of chromosomes do not code for proteins.
B: All of the DNA of chromosomes code for proteins.
C: different chromosomes have the same number of genes.
D: different chromosomes containt the same number of DNA bases.

Any help would be appreciated.

I have 2 questions for my homework on recombinant DNA. We made a little thing where we cut fake DNA and plasmids with a restriction enzyme. We had to pick the correct enzyme that had 1 cut in the plasmid and 2 cuts in the DNA and they had to not be in the shaded areas of the base pairs.

The 2 questions are asking why we shouldn’t cut in the shaded areas. I’m not sure what the shaded base pairs stand for. Could someone tell me why?

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

Radioactive or fluorescent-labeled RNA or single-stranded DNA pieces that are complementary to the gene of interest and are used to confirm the presence of a cloned gene are called
A. probes.
B. plasmids.
C. vaccines.
D. clones.

Which of the following procedures is not a usual step in a genetic engineering experiment?
A. inducing a mutation in a source chromosome
B. cleaving DNA with a restriction enzyme
C. recombining pieces of DNA from different species
D. cloning and screening target cells

Please help, I’m having a hard time with this test!

Question 1 (Multiple Choice Worth 2 points)

In humans, hemophilia is a sex-linked recessive trait. If a man and woman have a son with hemophilia, which of the following must be true?

The father has hemophilia.

The mother has hemophilia.

The father carries at least one allele for hemophilia.

The mother carries at least one allele for hemophilia.

Both parents carry at least one allele for hemophilia.

Question 2 (Multiple Choice Worth 2 points)

Biotechnology is currently being used to effectively do which of the following?

Produce vaccines

Produce human gene products

Diagnose diseases

Only Choice 1 and 3 are correct

Choices 1, 2, and 3 are correct

Question 3 (Multiple Choice Worth 2 points)

What two enzymes are needed to produce recombinant DNA?

Polymerase and ligase

Restriction enzyme and ligase

Transcriptase and ligase

Restriction enzyme and reverse transcriptase

Ligase and reverse transcriptase

Question 4 (Multiple Choice Worth 2 points)

The “central dogma” of molecular genetics is that genetic information is coded in self-replicating DNA and undergoes unidirectional transfer to messenger RNAs in transcription which act as templates for protein synthesis in translation. The exception to part of this statement is:

retroviruses

tumor viruses

plasmids

restriction enzymes

gene cloning

Question 5 (Multiple Choice Worth 2 points)

In a Mendelian cross between two pea plants that are heterozygous for flower color, what is the probability that the offspring will inherit at least one dominant allele?

1

3/4

2/3

1/2

1/4

Question 6 (Multiple Choice Worth 2 points)

A couple has two children who both have blue eyes and blonde hair. Both parents are homozygous for blue eyes (bb), but the mother has brown hair (Hh) and the father is a blonde (hh). What is the probability that their next child will have blue eyes and brown hair?

1

1/2

1/4

1/8

1/16

Question 7 (Multiple Choice Worth 2 points)

Which of the following best describes the parents in a testcross?

Both individuals are heterozygous

Both individuals are homozygous

Both individuals have the recessive phenotype

One individual has the dominant phenotype and the other has the recessive phenotype

Both individuals have an unknown phenotype

Question 8 (Multiple Choice Worth 2 points)

Which of the following is true of the Law of Independent Assortment?

It states that the alleles at different loci separate independently of each other in a monohybrid cross.

It is the reason that the expected ratio in the F2 generation is 9:3:3:1.

It states that individuals have 2 alleles for each gene and gametes end up with only one of the two alleles.

It states that if an organism has two different alleles for a gene, only one allele has a noticeable effect on the organism’s appearance.

More than one of the above answers is correct.

Question 9 (Multiple Choice Worth 2 points)

A pair of homologous chromosomes

often have different lengths

often have different alleles

often have different genes for different traits

are separated into different daughter cells during the process of mitosis

would have both been inherited only from the mother or only from the father

Question 10 (Multiple Choice Worth 2 points)

Polygenic inheritance is best represented by which of the following?

Skin pigmentation in humans

ABO blood group in humans

White and purple flower color in pea plants

Colorblindness in humans

Pink petal color in snapdragons

Question 11 (Multiple Choice Worth 2 points)

In human blood groups, Rh positive is dominant over Rh negative. A woman with A positive blood has a son who is B negative and a daughter who is O positive. Which of the following represents a possible genotype for the mother?

IAi rr

IAi RR

IAIA RR

IAIA Rr

IAi Rr

Question 12 (Multiple Choice Worth 2 points)

Based on the data in the chart below, answer the question that follows. Assume that the wild type parental fly (gray body/normal wings) is heterozygous for the traits, and that the recessive parental fly (ebony body/vestigial wings) is homozygous recessive for the traits. A total of 2000 flies were counted.

What could be concluded about these genes?

They are sex-linked, and affect only males.

They are autosomal on separate chromosomes.

They are autosomal but at opposite ends of the same chromosome.

They are not affected by crossover events.

They are linked on the same chromosome and experienced a crossover event.

Question 13 (Multiple Choice Worth 2 points)

A student performed a bacterial transformation experiment, and introduced a plasmid into an E.coli suspension that contained a resistance gene to the antibiotic, erythromycin. The student plated the tra

To produce genetically engineered bacteria that make a human protein, which of the following steps does a scientist have to take first?
a. insert the human gene for the protein into a plasmid
b. use a restriction enzyme to cut out the gene from human DNA
c. Transform bacteria with the recombinant plasmid
d. extract the protein from the bacterial culture

THANKS SO MUCH!

1. Which of these is the major benefit of having insulin through biotechnology
a.It can be mass-produced
b.It is non-allergenic
c.It is less expensive
d.It is readily available

2.Diabetic individuals are injected with insulin. Which among these practices is considered traditional?
a.Sharing insulin from other human
b.Sharing insulin from animals
c.Taking insulin from dead human
d.Taking insulin from slaughtered animals

3.Genetic engineer need to isolate the insulin gene from it’s chromosome by using
a.DNA ligase
b.restriction enzyme
c.plasmid
d.virus

4.What will happen of the gene coded to produce insulin is not properly isolated in the process gene cloning?
a.Insulin could not be produce.
b.the product will cause allergenic reaction
c.Other proteins maybe produce
d. no recombinant DNA be formed

5.It is a substance that serves as an antigen that promotes active immunity without causing disease.
a.vaccine
b.antibiotic
c.insulin
d.hormone