Heres the word bank:
GMO (Genetically Modified Organism)
size
plamid(s)
transgenic
DNA ligases
restriction enzyme(s)
gel electrophoresis
nucleic acid probe
genetic marker
PRC (Polymerase chain reaction)
recombinant DNA
animal/plant
plant/animal

AND HERE ARE THE FILL-INS

The lac repressor releases the operator in the presence of __________.

A DNA sample will form a single band on an electrophoresis gel if all the fragments are the same _________.

A protein produced by transgenic bacteria is identical to the same protein produced by humans because both are coded by __________.

THANK YOU SO MUCH IF YOU KNOW ANY/ALL!

a) is an amino acid

b) is present in the nucleus

c) moves specific amino acids to ribosomes to form protein

d) carries the genetic code from DNA to ribosomes

e) manufactures enzymes

I can’t get the answers to these. Can you help me out?! Thanks so much in advancee!

Molecules produced by combining DNA from different species
_ _ _ _ e _ _ _

Technique that uses a microscopic class needle to insert recombinant DNA into cells
_ _ _ _ _ _ _ _ _ _ t _ _ _

Process in which DNA pieces are seperated by an electric field
_ _ _ _ _ _ _ _ _ _ _ e _ _ _

Phenomenon in which products of a hybrid cross are hardier than either of the species that produced them
_ _ _ _ _ _ _ _ g _ _

Technique that identifies and replicates the noncoding gene sequences in a strand of DNA
_ _ _ _ _ _ _ _ _ _ _ i _ _ _ _ _

Protein that blocks the growth of viruses
_ n _ _ _ _ _ _ _ _

Inheritable changes in DNA
_ _ _ _ _ _ _ n _

a) is present in the nucleus
b) is an amino acid
c) moves specific amino acids to ribosomes to form protein
d) carries the genetic code from DNA to ribosomes
e) manufactures enzymes

Proteins are manufactured from "blueprints" found on DNA. After they are translated, they are moved through a system of internal membranes before being distributed throughout the rest of the cell. At some point in this process, they are modified to their functional form.

a) Describe the movement of proteins from their manufacturing point through this internal membrane system.

b) In your own words, discuss the role of each of the following structures in the whole process: cell membrane, vesicles (at two points in the process), ribosomes, Golgi apparatus, and endoplasmc reticulum. Be sure to identify the correct order in which the proteins move through each of these structures.

Transcription which is DNA to RNA and then translation which is RNA to protein, but I’m not understanding how to answer these questions.

Any help would be appreciated. =]
Sorry for the topic mess up. It’s supposed to read I need help understanding how proteins are made.
Erika- I’m asking all of that. =]

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

In our lab we are refolding recombinant Human growth hormone from E.Coli . in that urea and Gunadium Hcl is being used why do one should use urea or guanidium HcL in invitro refolding of recombinant therapeutic protein ? can any one give the exact molarity of Urea or Gunadium Hcl to be used for refolding ? which of the following either urea or Gunadium Hcl is best for refolding ?

Thanks so muchh in advance!

I can’t get the answers to these. Can you help me out?! Thanks so much in advancee!

Molecules produced by combining DNA from different species
_ _ _ _ e _ _ _

Technique that uses a microscopic class needle to insert recombinant DNA into cells
_ _ _ _ _ _ _ _ _ _ t _ _ _

Process in which DNA pieces are seperated by an electric field
_ _ _ _ _ _ _ _ _ _ _ e _ _ _

Phenomenon in which products of a hybrid cross are hardier than either of the species that produced them
_ _ _ _ _ _ _ _ g _ _

Technique that identifies and replicates the noncoding gene sequences in a strand of DNA
_ _ _ _ _ _ _ _ _ _ _ i _ _ _ _ _

Protein that blocks the growth of viruses
_ n _ _ _ _ _ _ _ _

Inheritable changes in DNA
_ _ _ _ _ _ _ n _

I can’t get the answers to these. Can you help me out?! Thanks so much in advancee!

Molecules produced by combining DNA from different species
_ _ _ _ e _ _ _

Technique that uses a microscopic class needle to insert recombinant DNA into cells
_ _ _ _ _ _ _ _ _ _ t _ _ _

Process in which DNA pieces are seperated by an electric field
_ _ _ _ _ _ _ _ _ _ _ e _ _ _

Phenomenon in which products of a hybrid cross are hardier than either of the species that produced them
_ _ _ _ _ _ _ _ g _ _

Technique that identifies and replicates the noncoding gene sequences in a strand of DNA
_ _ _ _ _ _ _ _ _ _ _ i _ _ _ _ _

Protein that blocks the growth of viruses
_ n _ _ _ _ _ _ _ _

Inheritable changes in DNA
_ _ _ _ _ _ _ n _