I think the answer is E, but I’m not sure.

An insertion sequence:

a) carries a gene only for transposase between two invertetd repeats
b)may transpose genes for antibiotic resistance to R plasmids
c) involves the exchange of homologous regions of DNA
d)is nessecary for the F plasmid to incorporate into the bacterial chromosome to form an Hfr cell
e) cosists of an inverted repeat sandwhiched between two direct repeats

(im not trying to ripp off answers here, I just need help)

Technorati Tags: antibiotic resistance, bacterial chromosome, dna, Genes, insertion, plasmid, Plasmids, transposase

A plasmid is a circular DNA molecule that scientists use to transform bacteria and plants.
 
Plasmids are naturally found in some ____________________.

a.
insects
c.
plants
b.
bacteria
d.
humans

Technorati Tags: bacteria, circular dna molecule, insects, plants, plasmid, scientists

I am working on this particular project but I do not really understand the following.

I am supposed to clone an N and a C terminus of GFP protein into a particular host cell. I am given those 2 plasmids and am asked to grow in bacteria first and then amplify it through PCR.

I am just wondering why shouldn’t I just amplify it by either PCR or bacteria. I know both have its pros and cons but why do you have to amplify it in 2 different ways? Wouldn’t you be losing money on that?
I do not want the plasmid at the end of the day. Why do I still need to transfect with bacteria then?

Technorati Tags: bacteria, c terminus, different ways, host cell, money, plasmid, Plasmids, pros and cons, protein

a. Those bacteria that contain the plasmid will survive.
b. The penicillin will kill the bacteria that were transformed.
c. The gene for antibiotic resistance is expressed in the bacteria that survive.
d. Those bacteria that are successfully transformed will survive.

HELP!!!!!!! :/

Technorati Tags: antibiotic resistance, bacteria, penicillin, plasmid

In genetic modification of bacteria, you cut out the desired gene, and you place it in the plasmid, and then you put the plasmid back into the bacteria.
My teacher told me that there are no plasmids in yeast, so where would you put the desired gene in a yeast cell?

Technorati Tags: bacteria, genetic modification, plasmid, Plasmids, yeast cell

we had to digest 3 samples of plasmids.
one with Ava ii
one with Pvu ii
and one with both ava ii and pvu ii

for ava ii i got 2 bands
pvu i got 4 bands
pvu/ava i got 5 bands
does anyone know what this means?

and what is the significance of running the uncut plasmid in the gel as well?

Technorati Tags: ava, plasmid, pvu, what this means

To "clone a gene," a DNA fragment containing the gene of interest is isolated from chromosomal DNA using restriction enzymes and then united with a plasmid that has been cut with the same restriction enzymes. When the fragment of chromosomal DNA is joined with its cloning vector in the lab, it is called a "recombinant DNA molecule." Following introduction into suitable host cells, the recombinant DNA can then be reproduced along with the host cell DNA.

I don’t know what some of the words mean , so …. yeah .

Technorati Tags: chromosomal dna, cloning vector, dna fragment, dna molecule, host cell, host cells, plasmid, recombinant dna, restriction enzymes, suitable host

A) plasmids are unable to replicate in cells.

B) only one copy of a plasmid can be present in any given cell, whereas many copies of a YAC or BAC can coexist in a single cell.

C) YACs and BACs can carry much larger DNA fragments than ordinary plasmids can.

D) YACs and BACs can be used to express proteins encoded by inserted genes, but plasmids
cannot.

E) all of these

Technorati Tags: bacs, cell c, cells, dna fragments, Genes, plasmid, Plasmids, proteins, yac, yacs

can someone please help me summarize this into different words please? thanks!

Expiramental procedures have been developed that allow recombinant DNA molecules to be engineered into a test tube. From a wide variety of retriction enzymes available, scientists choose one or two that recognize particular sequences of DNA within a longer DNA sequence of a chromosome. The enzymes are added to th DNA, Which is cleaved at the recognition sites. Because the cleaved fragments have ends that are available for attachments to complimentary strands, the fragments can be added to the plasmids or to viral DNA that has been similarly cut. When the DNA fragment has been incorporated into the plasmid or virus, it is called recombinant DNA.

Technorati Tags: attachments, choose one, chromosome, dna fragment, dna sequence, enzymes, fragments, plasmid, Plasmids, recombinant dna molecules, scientists, sequences, strands, test tube, viral dna, virus

ok, i have bioshock and i got the fire plasmid and the electric plasmid. but then when i find the telekiness i have to replace one of my plasmid. i only got 2 open slots and there are 5 in all. how can i unlock the other slots to hold more power-ups instead of swaping them? i dont want to switch them

Technorati Tags: bioshock, open slots, plasmid, power ups, ups