Nature Technology Corporation » plasmid

A plasmid has two antibiotic restriction genes..?

NTC — Mon, 26 Jul 2010 17:01:13 +0000

A plasmid has two antibiotic restriction genes, one for ampicillin and one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a human globin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase, and used to transform bacteria cells. Clones that have taken up the recombinant DNA are ones that:

A. are blue and can grow on plates with both antibiotics
B. can grow on plates with ampicilin but not with tetracycline
C. can grow on plates with tetracycline but not with ampicillin
D. cannot grow with any antibiotics
E. can grow on plates with tetracycline and are blue.

A plasmid has two antibiotic restriction genes..? is a post from: Nature Technology Corporation

Technorati Tags: ampicilin, ampicillin, antibiotics, bacteria, cells, clones, Genes, globin gene, ligase, plasmid, Plasmids, recombinant dna, restriction enzyme, tetracycline

Are R-Plasmids mobile?

NTC — Tue, 20 Jul 2010 18:00:33 +0000

actually i’ve been struggling with this question which has got two correct answers: "what are mobile sequences in bacteria?
a) lysogenic phage
b)f-plasmid
c)promoters
d)r-plasmids
e)temperate phage"
and i’d like to know if the R-Plamids are mobile or not?
thanks

Are R-Plasmids mobile? is a post from: Nature Technology Corporation

Technorati Tags: bacteria, correct answers, lysogenic, plasmid, Plasmids, promoters, quot, sequences

is it a,b,c,d…?

NTC — Sun, 27 Jun 2010 16:00:54 +0000

soppose a bacterial culture were mixed with recombinant plasmids containing a gene for resistance to penicillin. the bacterial culture was then treated with penicillin. which of the following statements is NOT true?

a.those bacteria that contain the plasmid will survive.

b.the penicillin will kill the bacteria that were transformed.

c.the gene for antibiotic resistance is expressed in the bacteria that will survive.

d.those bacteria that are transformed will survive.

is it a,b,c,d…? is a post from: Nature Technology Corporation

Technorati Tags: antibiotic resistance, bacteria, bacterial culture, penicillin, plasmid, recombinant plasmids

Huge lab in chemistry I need help on…?

NTC — Sat, 26 Jun 2010 16:48:45 +0000

We’re doing this lab called an amgen lab where you take the genetic information in bacteria called a plasmid and you put it in another plasmid. It’s kind of like two donuts switching pieces. So, after we did the electrophoresis (it’s where you put the plasmids in a machine that puts electricity through it to see how charged the particles are) and we have to answer questions. We have to examine the data from the electrophoresis and the question is "Is there evidence of more than one form of multimer?" What the heck does that mean? I need an answer from a chemist or something that understands what the heck I’m doing. If you can’t answer this question, if you understand the slightest bit what I’m talking about, can you explain it to me? I need serious help. If you don’t even think I’m speaking english don’t even bother to answer. Thanks.

Huge lab in chemistry I need help on…? is a post from: Nature Technology Corporation

Technorati Tags: amgen, bacteria, chemist, donuts, electricity, electrophoresis, genetic information, particles, plasmid, Plasmids, speaking english, what the heck

biology questions! :)?

NTC — Mon, 21 Jun 2010 15:25:34 +0000

A strand of DNA formed by the splicing of DNA from two different species is called ___________.
Choose one answer.

a. determinant DNA

b. recombinant DNA

c. plasmid

d. restriction DNA

A family has four girls and two boys. Both of the boys have hemophilia. The parents go to see a genetic counselor, which tells them that hemophilia is carried on the X chromosome. This is an example of __________.
Choose one answer.

a. a sex-linked trait

b. incomplete dominance

c. codominance

d. dominance

DNA fingerprinting uses the fact that individuals have different numbers of ___________.
Choose one answer.

a. genes in their Y chromosomes

b. genes in their X chromosomes

c. repeating units

d. chromosomes

Which of the following is used to make crop plants resistant to herbicides?
Choose one answer.

a. Ti plasmids

b. pellets

c. plasmids

d. nodules

biology questions! :)? is a post from: Nature Technology Corporation

Technorati Tags: codominance, crop plants, determinant, different numbers, dna c, four girls, genetic counselor, hemophilia, herbicides, incomplete dominance, nodules, pellets, plasmid, Plasmids, recombinant dna, sex linked trait, two boys, x chromosome, x chromosomes, y chromosomes

Biology help :) please ASAP?

NTC — Sun, 20 Jun 2010 16:25:16 +0000

1.What are the two types of vectors used in recombinant DNA technology, and give short examples of each please.
2. How are restriction enzymes important tools in genetic engineering?
3. Why is it essential that both the plasmid and the donor DNA be exposed to the same restriction enzyme?
4. What must researchers know before they begin the process of Genetic engineering?
5. Why does producing a single bacterium through genetic recombinant enable researchers to produce large numbers of bacteria with the recombined plasmid?
6. What would be the goal of a researcher who inserted the gene for insulin onto bacteria?
Thanks! I was out of school for three days and have 7 biology worksheets now!

Biology help :) please ASAP? is a post from: Nature Technology Corporation

Technorati Tags: bacteria, bacterium, biology, donor dna, important tools, insulin, large numbers, plasmid, process of genetic engineering, recombinant dna technology, researcher, restriction enzyme, restriction enzymes, vectors

Need help with GENETICS! please?

NTC — Fri, 18 Jun 2010 17:04:41 +0000

You have successfully cloned gene RA into your chosen vector. What can you now do with gene RA? (Mark all that apply)
1.Sequence gene RA to determine if there are any mutations in the original organism
2.Transform plasmid into bacteria and investigate RNA splicing
3.Mutate gene RA to determine specific amino acids that are critical to protein function
4.Transform plasmid into bacteria and isolate translated RA protein
5.Conjugate plasmid into bacteria and isolate gene RA transcribed RNA

Need help with GENETICS! please? is a post from: Nature Technology Corporation

Technorati Tags: amino acids, bacteria, conjugate, mutations, organism, plasmid, protein function, ra, rna splicing, vector

Plasmid curing activity of five plant extracts on multiple resistant plasmid bearing Klebsiella Pneumoniae strains

admin — Wed, 16 Jun 2010 07:33:34 +0000

Plasmid curing activity of five plant extracts on multiple resistant plasmid bearing Klebsiella Pneumoniae strains

Abstract:

The curing of five medicinal plant extracts (Satureia hortensis, Gly cyrrhiza glabra, Teucrium Pollium, Myrtus Communis, Eucalyptus globolus) on plasmid bearing strains of klebsiella pneumoniae resistance to multiple antibiotics were investigated. Alcoholic and maceration extraction were carried out from leaves, fruits or roots of the above plants. Minimum inhibitory concentrations (MIC) of each plant extract indicated that all of them have antimicrobial activity and were able to inhibit the growth of resistant strains of k. Pneumoniae. Furthermore the eucalyptus extract had highest antimicrobial activity as compared to other plant extracts (Average MIC 0.1 mg/ml). At the same time the subinhibitory concentration (SIC) and minimum bactericidal concentration (MBC) activity of each plant extract was carried out in order to cure plasmids from plasmid bearing strains. Curing experiments were performed from SIC tubes which then diluted serially (10-2, 10-4, 10-6, 10-8) and 100 well isolated colonies were then master plated with sterile tooth pikes. The sensitivity of the strains treated with SIC of each plant extracts to Ampicillin Amoxicillin, cefotaxime, and Ceftizoxime indicated that there was no change in MIC of antibiotics compared to unexposed one. The morphological investigation with fluorescent microscope (Nikon, optiphota-2) and capsule staining indicated that when cells treated with Myrtus Communis extract, the cells aggregated and change their morphology, similarly, production of cupsule was also lost. The agarose gel electrophoresis (0.7%) of the treated cells and untreated one and observation of the gel with U.V. gel documentation system indicated that there were any physical losses of plasmid band on the gel. Similarly, the synergistic action of plant extract and antibiotics were investigated and it was found that these were no synergy between the above plant extracts and antibiotics (MIC remained unchanged). From above data it could be concluded that the above plants extract though they have antibacterial activity they did not exert any curing effect on the plasmid bearing microorganisms isolated from hospitals in Kerman Iran.

Plasmid curing activity of five plant extracts on multiple resistant plasmid bearing Klebsiella Pneumoniae strains is a post from: Nature Technology Corporation

Technorati Tags: activity, bearing, curing, extracts, five, Klebsiella, multiple, plant, plasmid, Pneumoniae, resistant, strains

how are viruses used for gene therapy/vectors?

NTC — Tue, 15 Jun 2010 16:04:00 +0000

So I read wikipedia etc but I don’t understand it in simple terms.

Here are the notes from biology class- could one of you smart biologists explain this even more simply

-Biologists use the capacity of parvoviruses to integrate into host genomes to deliver ‘transgenes’. You clone a gene of interest on a plasmid, express that plasmid in "packaging cells
that also express parvovirus capsid proteins and the required adenovirus "help.". You produce parvovirus particles capable of delivering the transgene to infected cells, where it will integrate. Transgene will be expressed in those cells.

how are viruses used for gene therapy/vectors? is a post from: Nature Technology Corporation

Technorati Tags: adenovirus, biologists, biology class, capsid proteins, cells, genomes, particles, parvovirus, parvoviruses, plasmid, transgene, wikipedia

Biology question.. transformed bacteria?

NTC — Tue, 08 Jun 2010 16:39:10 +0000

Suppose a bacterial culture were mixed with recombinant plasmids containing a gene for resistance to penicillin. The bacterial culture was then treated with penicillin. Which of the following statements is NOT true?

a.
Those bacteria that contain the plasmid will survive.
b.
The penicillin will kill the bacteria that were transformed.
c.
The gene for antibiotic resistance is expressed in the bacteria that survive.
d.
Those bacteria that are successfully transformed will survive.

Biology question.. transformed bacteria? is a post from: Nature Technology Corporation

Technorati Tags: antibiotic resistance, bacteria, bacterial culture, penicillin, plasmid, recombinant plasmids