In a hypothetical situation, the genes for sex pilus construction and for tetracycline resistance are located together on the same plasmid within a particular bacterium. If this bacterium readily performs conjugation involving a copy of this plasmid, then the result should be..

A) the production of endospores among the bacterium’s progeny.
B) the rapid spread of tetracycline resistance to other bacteria in that habitat.
C) the temporary possession by this bacterium of a completely diploid genome.
D) a transformed bacterium
E) the subsequent loss of tetracycline resistance from this bacterium.

Why are circular plasmids capable of transforming E coli and being stably maintained? What features of a plasmid allow this to happen?

1. which statement is not a characteristic of plasmids
a. extra-chromosomal
b. replicate independently
c. easily moved out of, and into, bacterial cells
d. used as DNA delivery units in biotechnology
e. the same size as bacterial chromosomes

2. which of the following is the most commonly used cloning vector in genetic engineering

3. Bacterial transformation is a bacterial ability to
a. cut foreign DNA
b. produce proteis from another organism’s genes
c. take up DNA from its surroundings.
d. connect DNA fragments once they are cut with restriction enzymes
e. produce restriction enzymes

4. what is a cloning vector?
a. a cell that is producing protein from a reombinant plasmid
b. a DNA vehicle that carries recombinant DNA into cells
c. an enzyme used to cut or combine DNA
d. a protein like HIGH that is produced by biotech.
e. an animal that is an exact copy of another animal

In a hypothetical situation, the genes for sex pilus construction and for tetracycline resistance are located together on the same plasmid within a particular bacterium. If this bacterium readily performs conjugation involving a copy of this plasmid, then the result should be

a transformed bacterium.
the production of endospores among the bacterium’s progeny.
the subsequent loss of tetracycline resistance from this bacterium.
the temporary possession by this bacterium of a completely diploid genome.
the rapid spread of tetracycline resistance to other bacteria in that habitat.

I’m doing a report on a journal article Model Structure of Human APOBEC3G and under the subheading of Mutation analysis, constructs, viral production and titration the first sentence reads: "The plasmid expressing a hemagglutinin (HA)-tagged form of APOBEC3g was a kind gift from M. Malim"
You can find the article online at: http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0000378;jsessionid=CDDDA897E73BDE4991DB33929995FA10

are located together on the same plasmid within a particular bacterium. If this bacterium performs conjugation involving a copy of this plasmid, then the result should be

a. a transformed bacterium
b. the rapid spread of tetracycline resistance to other bacteria in that habitat
c. the subsequent loss of tetracycline resistance in this bacterium
d. the production of endospores among the bacterium’s progeny
e. the temporary possession by this bacterium of a completely diploid genome

I have the restriction sequences for four restriction endonucleases, and its asking which pair of restriction endonucleaeses i would use to digest the plasmid and the foreign DNA. How do i know which ones to use and why?

It says to "describe how a gene of interest can be inserted into a plasmid, how the plasmid could then be transferred into a bacterial cell, and how the recombinant bacteria could be identified. I’ve searched for stuff but can’t really find anything, any help would be greatly appreciated.

Part 1= Answers

1. What is the study of all of an organism’s proteins?

2. What is bacterial enzyme that recognizes and binds to specific short sequences of DNA, then cuts DNA at specific sites within the sequence.

3. What is a technique that uses an electrical field within a gel to separate molecules by their size and charge?

4. What is a circular DNA molecule found naturally in some bacteria and that can replicate independently of the main chromosome of bacteria?

5. What is and agent that is used to carry the gene of interest into another cell?

6. What is DNA made from two or more different organisms?

7. What is the process of altering genetic material to produce new substances?

Part 2= Matching

1. Genetic Engineering
2. Vector
3. Plasmid
4. Cloning
5. Recombinant DNA
6. Restriction Enzymes
7. Gel Electrophoresis

A. made from DNA from two separate organisms.
B. uses an electrical field to separate molecules
C. growing a large number of genetically identical cells from a single cell.
D. alters an organism’s DNA so it codes for new substances.
E. can carry a DNA fragment into another cell.
F. can be used as a vector.
G. used to cut DNA at specific sequence.

Please i need this for at the least tomorrow night. Thanks so much!

If you transform a plasmid that has a colicin gene to Pseudomonas …. How do you know that the plasmid if the plasid is producing this protein ir not