Nature Technology Corporation » green fluorescent protein

The gene carried by the plasmid which is inserted into the bacterium in this experiment, codes for which of t?

NTC — Wed, 03 Mar 2010 18:55:53 +0000

1. enzyme
2. green, fluorescent protein
3. transport protein in the cell membrane
4. histone protein

Best answer awarded

The gene carried by the plasmid which is inserted into the bacterium in this experiment, codes for which of t? is a post from: Nature Technology Corporation

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“Earth BIOLOGY quiz” can you help me?

NTC — Tue, 02 Feb 2010 00:06:08 +0000

1.How was the gene in this species of fruit fly tagged for study?
A. Radioactive glucose
B. Green fluorescent protein genes
C. Carbon 14
D. Cobalt 60

2.Bees use the _____ to find flowers reported by forager bees.
A. position of sun
B. magnetic field
C. aroma gradient
D. all of the above

3. If the direction of the dance is toward the upper part of the hive then the flowers are _____.
A. in the direction of the sun
B. at a 90 degree angle to the sun
C.. at a 45 degree angle to the sun
D. all of the above

4.The duration of the dance and the number of vibrations is used to communicate the _____ to flowers.
A. direction
B. distance
C. type of nectar
D. all of the above

5.Bees take account of the ____ when communicating the direction of flowers.
A. wind
B. relative hunidity
C. movement of the sun
D. all of the above

“Earth BIOLOGY quiz” can you help me? is a post from: Nature Technology Corporation

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Easy multiple choice biology.?

NTC — Sun, 31 Jan 2010 17:22:42 +0000

Easy multiple choice questions for easy points!

1.The technique that showed the arrangement of the two layers making up the cell membrane was
a.fluorescence microscopy.
b.confocal laser technology.
c.transmission electron microscopy.
d.light microscopy of stained sections.

2.The technique that gives information on the three-dimensional structure of molecules is
a.X-ray crystallography.
b.confocal laser technology.
c.scanning electron microscopy.
d.green fluorescent protein technology.

Easy multiple choice biology.? is a post from: Nature Technology Corporation

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Is it possible to genetically engineer an oak tree with glowing leaves?

NTC — Sun, 31 Jan 2010 11:23:26 +0000

A couple months ago I went on a BioBridge Trip where we genetically engineered e. coli with the green fluorescent protein found in jellyfish. In my bio class we are also about half way through genetics. Anyway, on this biobridge trip it says a scientist, who’s name I forget, mutated the GFP (Green Fluorescent Protein) gene so that it would generate different colors (grape, cherry, tangerine, etc.). Anyway, I was wondering if it was possible to take the gene that creates luciferase in fireflies and mutate it to be other colors. Also, I was wondering if you could make say an oak tree that’s leaves produced luciferase or a rose bush with glowing flowers? I know that all cells in an organism posess the full genetic code of that organism, could the leaves or flowers be made so that only they express that gene. For those that don’t know much about science and dismiss the thought right away here’s a tobacco plant that produces luciferase: http://campus.queens.edu/faculty/jannr/Genetics/images/dnatech/fluorescentTobacco.jpg

Is it possible to genetically engineer an oak tree with glowing leaves? is a post from: Nature Technology Corporation

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Reporter Gene?

NTC — Sat, 30 Jan 2010 16:10:30 +0000

4.Reporter genes such as those for green fluorescent protein (GFP) are found on many commercial plasmids. These genes are useful to determine:
a.if the plasmid is in the host.
b.if a gene is present in a library.
c.if a foreign protein is being expressed on a vector.
d.the relative strength of a promoter sequence.
e.all of the above.

Could you give an explaination too? I don’t just want to know the answer, I want to understand it

Reporter Gene? is a post from: Nature Technology Corporation

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Molecular biology?

NTC — Sat, 30 Jan 2010 10:10:02 +0000

When do i used the following methods. Why would i use one method instead of the other? are they all used in determining gene expression?
Methods
1. in situ hybridization
2. northern blotting
3. antibody staining
4. green fluorescent protein
5. Lac z ( b-galactosidase)
6. fluorescent antibody labeling
7. nucleic acid hybridization
8. genome sequence.

Molecular biology? is a post from: Nature Technology Corporation

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Steps in producing GFP?

NTC — Sat, 30 Jan 2010 04:45:58 +0000

The laboratory goal was to use restriction digest and PCR to distinguish two plasmids: pGLO and pBR322. The gfp gene has been sequenced and cloned into pGLO plasmid. I have identified which plasmid is which. What is the next step to create Green Fluorescent Protein?

Steps in producing GFP? is a post from: Nature Technology Corporation

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pGLO transformation of Escherichia coli~~~ questions!?

NTC — Fri, 29 Jan 2010 11:22:51 +0000

1) What is the source of the GFP (green fluorescent protein) gene?

2) what is pGLO DNA?

3) What is genetic transformation? List the three genes involved in the pGLO transformation of Escherichia coli and the trait they each give the bacteria.

4) what is the purpose of adding CaCl2 followed by heat shock?

5)what is the role of the sugar, arabinose in the transformation of Escherichia coli bacterium?

6) If there are any genetically transformed bacterial cells, on which plates would you expect to find them? Explain.

pGLO transformation of Escherichia coli~~~ questions!? is a post from: Nature Technology Corporation

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i don’t get what this question is trying to say in my biology hw?

NTC — Thu, 28 Jan 2010 13:16:20 +0000

here is the question

provide a rational or benefit of adding DNA sequences coding for fluorescent proteins such as Green Fluorescent Protein, to tag genes of interest in plasmids used for transformation.

i don’t get what this question is trying to say in my biology hw? is a post from: Nature Technology Corporation

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Any suggestions for research paper?

NTC — Wed, 27 Jan 2010 19:47:22 +0000

I have to do a 10 page research paper that has to do with science but I can’t write about something broad. I was looking into writing something about genetics, green fluorescent protein, or memory. I don’t know what to choose and what I could write about in 1 of them to make it narrow.. any suggestions or help? :/

Any suggestions for research paper? is a post from: Nature Technology Corporation

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