using E.coli containig plasmids DNA carrying gene for GH, DNA ligase a restriction enzyme and growth medium

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

"You are attempting to introduce a gene that imparts larval moth resistance to bean plants. Which of the following vectors are you most likely to use?"

a. phage DNA
b. E. coli Plasmid
c. Ti plasmid
d. yeast plasmid
e.bacterial artificial chromosome

my book stipulates nothing about these and I’m lost :[[

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

a. compatible human cells.
b. antibiotic-sensitive E. coli cells that become antibiotic-resistant if transformed
c. bacgteriophages
d. mRNA molecules

In our lab we are refolding recombinant Human growth hormone from E.Coli . in that urea and Gunadium Hcl is being used why do one should use urea or guanidium HcL in invitro refolding of recombinant therapeutic protein ? can any one give the exact molarity of Urea or Gunadium Hcl to be used for refolding ? which of the following either urea or Gunadium Hcl is best for refolding ?

Why are circular plasmids capable of transforming E coli and being stably maintained? What features of a plasmid allow this to happen?

A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.

First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds

I did a phenol chloroform extraction and an ethanol precipitation ( started with E coli culture containing my plasmid – was in YT broth).

The photo of my gel shows a blob at the end of the lane( furthest from well) what impurities would be in this and how is it best to remove them?

Many thanks

9. A search for sequences that are complementary to the desired sequence of a DNA fragment uses a technique called
A. plasmid insertion
B. vector extraction
C. cloning
D. electrophoresis
E. hybridization
10. All of the following involve genetic engineering techniques except
A. cutting and rearranging the DNA
B. using restriction enzymes to cut specific
sequences of DNA
C. cloning the genes into the host organism
D. using any cell as a vector
E. only a, b, and c are correct
11. A plasmid must have an origin of replication to allow it to replicate in E. coli independently of the chromosome and
A. a phage capable of infecting the E. coli bacterium
B. a tissue plasminogen activator
C. multiple cloning sites
D. a selectable marker, usually antibiotic resistance
E. only a, b, and c are correct
12. DNA fragments complementary to the DNA being investigated are referred to as
A. rDNA
B. cDNA
C. mDNA
D. tDNA