John Smith asked:
"steps in DNA isolation?"
This i a little bit of a vague question, but it is more specific than some questions I have seen. Why was it removed? I have read the community guidelines and did not see anything that would not prohibit this question? I am just curious.
Describe the main function of each of the following solutions used in plasmid DNA isolation
(i) Resuspension buffer
(ii) Lysis buffer containing a detergent
(iii) Neutrlization buffer with an acid component
(iv) Chloroform
What size and copy number would be most ideal for a plasmid to be a useful cloning vector?
Besides alkaline denaturation, name one other approach which can be used to isolate plasmid DNA from E coli.
Is using E Coli based plasmid vector to express a human gene in E coli ideal? Explain.
Please help!!
DNA isolation by inorganic method
in DNA isolation procedure ,after adding protease …why we are keeping it at 50 degree celsius?
Im doing research on DNA isolation and im having a really hard time finding information on it. I keep getting sites on DNA Extraction. Are they they same thing? Help?!
in my practicals i am preparing agarose gel in that i am adding low eeo agarose. what is electroendoosmosis(eeo)? what is high eeo agarose and its uses and medium eeo agarose uses? why we go with low eeo agarose in dna isolation.
Why are sterile techniques used in DNA isolation?
What are some advantages for using E. coli as an experimental oraganism?
How does a change in genotype result in a change in phenotype?
thank you!!!!!!
