Nature Technology Corporation » dna isolation

Quantifying Genomic and Organelle DNA in Isolation?

NTC — Mon, 14 Jun 2010 22:00:18 +0000

I have a protocol for nuclear DNA isolation, and would like to ensure that there is no contamination from organelle DNA. Is there a quick and easy way of quantifying nuclear DNA versus organelle? DNA?

Quantifying Genomic and Organelle DNA in Isolation? is a post from: Nature Technology Corporation

Technorati Tags: contamination, dna isolation, nuclear dna, organelle, protocol

DNA won't dissolve after isolating! In both TE and nuclease-free water?

NTC — Thu, 03 Jun 2010 15:14:31 +0000

After I complete my DNA isolation, I find it very hard to dissolve the DNA pellet into solution. I performed a trizol isolation on HMVEC cells and I’ve tried using both TE and nuclease-free water to dissolve it, but neither works. I’ve also tried leaving it out overnight at room temperature, leaving it in 4* overnight and mixing, and have tried heating it. Anyone have any suggestions?
I know redoing it is probably the easiest way, but I’ve noticed this happens a lot during DNA isolation. I usually work with RNA and don’t have a problem, but DNA is tough. I only dry the vials for a couple of minutes after the ethanol step so that the pellet doesn’t over-dry, but I keep getting the same result.

DNA won't dissolve after isolating! In both TE and nuclease-free water? is a post from: Nature Technology Corporation

Technorati Tags: cells, couple of minutes, dna isolation, ethanol, free water, rna, room temperature, vials

what is the difference between RNA and DNA that allows them to be separated?

NTC — Mon, 12 Apr 2010 23:02:35 +0000

what is the difference between RNA and DNA that allows them to be separated during DNA isolation??

what is the difference between RNA and DNA that allows them to be separated? is a post from: Nature Technology Corporation

Technorati Tags: dna isolation, rna and dna

why in isolation of dna we are using low EEO agarose?

NTC — Thu, 11 Feb 2010 18:12:21 +0000

in my practicals i am preparing agarose gel in that i am adding low eeo agarose. what is electroendoosmosis(eeo)? what is high eeo agarose and its uses and medium eeo agarose uses? why we go with low eeo agarose in dna isolation.

why in isolation of dna we are using low EEO agarose? is a post from: Nature Technology Corporation

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Can someone help me with sentence structure for an application essay?

NTC — Thu, 04 Feb 2010 14:28:02 +0000

Okay, so I am writing an essay to get into a Biotechnology Camp.
I have to write two essays an I need help with a few things

1) I hope to excel "into" advance placements courses such as….
Shoud it be into or in ( presently im not in any AP courses)

2) I am expecting this program to be a prolific and enjoyable experience for my ……..
What is another word for prolific except productive

3) This camp introduces various techniques in modern biotechnology research such as…..

What techniques should I put after "such as" that are specific to biotechonology or are just fit all. Below are a list of laboratory techiniques that is going done in this camp:

Laboratory Activities: Students will explore topics in three general areas of science: microbiology, biochemistry and molecular biology. Activities will include: growth, isolation and characterization of microbes, protein chemistry, chromatography, enzy-mology, regulation of gene expression, DNA isolation analysis, amplification (PCR) and genetic modification using standard laboratory model organisms (bacteria, yeast, Dictyostelium, Euglena, C. elegans).

Can someone help me with sentence structure for an application essay? is a post from: Nature Technology Corporation

Technorati Tags: activities students, amplification, ap courses, biochemistry and molecular biology, biology activities, biotechnology research, c elegans, chromatography, dna isolation, enjoyable experience, euglena, genetic modification, laboratory activities, laboratory model, microbes, model organisms, pcr, protein chemistry, regulation of gene expression, writing an essay

karyotyping in plants?

NTC — Tue, 02 Feb 2010 19:59:30 +0000

Do u know any useful sites interesting in plant molecular procedures, DNA isolation from plant tissues, karyotyping, SSR analysis. thanks.

karyotyping in plants? is a post from: Nature Technology Corporation

Technorati Tags: dna isolation, plant tissues

Calculate the molar concentration of NaCl in the buffer.?

NTC — Tue, 02 Feb 2010 13:14:47 +0000

In order to produce a special buffer for DNA isolation experiment, 250 grams of NaCl was dissolved in 1500 mL of water. Calculate the molar concentration of NaCl in the buffer.

Calculate the molar concentration of NaCl in the buffer.? is a post from: Nature Technology Corporation

Technorati Tags: dna isolation, ml, molar concentration, special buffer

Are PCR mix and Master Mix the same thing? When do I add them?

NTC — Tue, 02 Feb 2010 09:00:11 +0000

I’ve been put in charge of genotyping some blood and I don’t have a protocol. My main question is, after DNA isolation and checking the concentration of DNA, do I then add the PCR mix and then the Master Mix? Are the PCR mix and the Master Mix the same thing? I know after that, I just go ahead, run the PCR, then run the gel, but I’m confused on those two steps. THANKS!

Are PCR mix and Master Mix the same thing? When do I add them? is a post from: Nature Technology Corporation

Technorati Tags: concentration, dna isolation, protocol, two steps

DNA fingerprinting can be used?

NTC — Mon, 01 Feb 2010 15:36:42 +0000

1.Which of the following is a proper base pair in DNA?
A. A-T
B. C-A
C. G-G
D. T-G
E. None of the above

2.DNA fingerprinting can be used on:
A. Plants
B. Bacteria
C. Igneous Rocks
D. A and B
E.All of the above

3.Which of the following represents the correct order of steps in DNA fingerprinting?
A. DNA isolation–>restriction enzyme digestion–>gel electrophoresis
B. Restriction enzyme digestion–>gel electrophoresis–>DNA isolation
C. DNA isolation–>gel electrophoresis–>restriction enzyme digestion
D. None of the above

4.During gel electrophoresis, a positive charge is applied to:
A. The top of the gel
B. The bottom of the gel
C. It does not matter

5.In an electrophoretic gel:
A. DNA bands near the top of the gel are the smallest DNA fragments
B. DNA bands near the top of the gel are the largest DNA fragments
C. You cannot tell the size of a DNA fragment based upon looking at the DNA bands

DNA fingerprinting can be used? is a post from: Nature Technology Corporation

Technorati Tags: bacteria, base pair, dna bands, dna fragment, dna fragments, dna isolation, electrophoretic, gel electrophoresis, igneous rocks, plants, restriction enzyme digestion

Onion DNA problem?

NTC — Mon, 01 Feb 2010 09:02:52 +0000

I gotta write a lab report for our onion dna isolation experiment. Since I am a foreigner, I can’t understand 2 questions very well,
1) What are the properties of DNA that allows this molecule to be isolated ?
2) Why is it possible to spool up a single molecule like DNA ?

I kno I should do it myself, but it is too complicated, i mean my english is not enuff to understand.

Onion DNA problem? is a post from: Nature Technology Corporation

Technorati Tags: dna isolation, foreigner, molecule, onion