in my practicals i am preparing agarose gel in that i am adding low eeo agarose. what is electroendoosmosis(eeo)? what is high eeo agarose and its uses and medium eeo agarose uses? why we go with low eeo agarose in dna isolation.
Okay, so I am writing an essay to get into a Biotechnology Camp.
I have to write two essays an I need help with a few things
1) I hope to excel "into" advance placements courses such as….
Shoud it be into or in ( presently im not in any AP courses)
2) I am expecting this program to be a prolific and enjoyable experience for my ……..
What is another word for prolific except productive
3) This camp introduces various techniques in modern biotechnology research such as…..
What techniques should I put after "such as" that are specific to biotechonology or are just fit all. Below are a list of laboratory techiniques that is going done in this camp:
Laboratory Activities: Students will explore topics in three general areas of science: microbiology, biochemistry and molecular biology. Activities will include: growth, isolation and characterization of microbes, protein chemistry, chromatography, enzy-mology, regulation of gene expression, DNA isolation analysis, amplification (PCR) and genetic modification using standard laboratory model organisms (bacteria, yeast, Dictyostelium, Euglena, C. elegans).
Do u know any useful sites interesting in plant molecular procedures, DNA isolation from plant tissues, karyotyping, SSR analysis. thanks.
In order to produce a special buffer for DNA isolation experiment, 250 grams of NaCl was dissolved in 1500 mL of water. Calculate the molar concentration of NaCl in the buffer.
I’ve been put in charge of genotyping some blood and I don’t have a protocol. My main question is, after DNA isolation and checking the concentration of DNA, do I then add the PCR mix and then the Master Mix? Are the PCR mix and the Master Mix the same thing? I know after that, I just go ahead, run the PCR, then run the gel, but I’m confused on those two steps. THANKS!
1.Which of the following is a proper base pair in DNA?
A. A-T
B. C-A
C. G-G
D. T-G
E. None of the above
2.DNA fingerprinting can be used on:
A. Plants
B. Bacteria
C. Igneous Rocks
D. A and B
E.All of the above
3.Which of the following represents the correct order of steps in DNA fingerprinting?
A. DNA isolation–>restriction enzyme digestion–>gel electrophoresis
B. Restriction enzyme digestion–>gel electrophoresis–>DNA isolation
C. DNA isolation–>gel electrophoresis–>restriction enzyme digestion
D. None of the above
4.During gel electrophoresis, a positive charge is applied to:
A. The top of the gel
B. The bottom of the gel
C. It does not matter
5.In an electrophoretic gel:
A. DNA bands near the top of the gel are the smallest DNA fragments
B. DNA bands near the top of the gel are the largest DNA fragments
C. You cannot tell the size of a DNA fragment based upon looking at the DNA bands
I gotta write a lab report for our onion dna isolation experiment. Since I am a foreigner, I can’t understand 2 questions very well,
1) What are the properties of DNA that allows this molecule to be isolated ?
2) Why is it possible to spool up a single molecule like DNA ?
I kno I should do it myself, but it is too complicated, i mean my english is not enuff to understand.
John Smith asked:
"steps in DNA isolation?"
This i a little bit of a vague question, but it is more specific than some questions I have seen. Why was it removed? I have read the community guidelines and did not see anything that would not prohibit this question? I am just curious.
Describe the main function of each of the following solutions used in plasmid DNA isolation
(i) Resuspension buffer
(ii) Lysis buffer containing a detergent
(iii) Neutrlization buffer with an acid component
(iv) Chloroform
What size and copy number would be most ideal for a plasmid to be a useful cloning vector?
Besides alkaline denaturation, name one other approach which can be used to isolate plasmid DNA from E coli.
Is using E Coli based plasmid vector to express a human gene in E coli ideal? Explain.
Please help!!
DNA isolation by inorganic method
