Nature Technology Corporation » dna extraction http://www.natx.com/topblog Just another WordPress weblog Thu, 29 Jul 2010 11:32:56 +0000 en hourly 1 http://wordpress.org/?v=3.0 Can someone explain this to me? Transgenic Organism? http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism-2 http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism-2#comments Wed, 02 Jun 2010 18:34:05 +0000 NTC http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism-2 Can someone explain this to me? Transgenic Organism? is a post from: Nature Technology Corporation

]]> Hey everyone!

At the moment, I am working on a project, but I cannot seem to understand something. In order to create a organism with a foreign DNA sequence, there is a method that must be followed. Correct? Example, if I wanted to grow antlers on a rabbit, I’d take the gene for antlers from a deer and place it into the rabbit’s DNA. Is this the correct method?

1) PCR
2) RFLP
3) Edman Method
4) Blotting
5) DNA Extraction
6) Plasmids
7) Vectors

If this is the correct sequence, can you please explain why each step is present? I understand that the PCR step is their to make copies of the DNA, but what about the RFLP and the others?

Thanks so much!

Can someone explain this to me? Transgenic Organism? is a post from: Nature Technology Corporation

Technorati Tags: , , , , , , , , ,

]]> http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism-2/feed 1 Can someone explain this to me? Transgenic Organism.? http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism#comments Sat, 22 May 2010 20:21:52 +0000 NTC http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism Can someone explain this to me? Transgenic Organism.? is a post from: Nature Technology Corporation

]]> Hey everyone!

At the moment, I am working on a project, but I cannot seem to understand something. In order to create a organism with a foreign DNA sequence, there is a method that must be followed. Correct? Example, if I wanted to grow antlers on a rabbit, I’d take the gene for antlers from a deer and place it into the rabbit’s DNA. Is this the correct method?

1) PCR
2) RFLP
3) Edman Method
4) Blotting
5) DNA Extraction
6) Plasmids
7) Vectors

If this is the correct sequence, can you please explain why each step is present? I understand that the PCR step is their to make copies of the DNA, but what about the RFLP and the others?

Thanks so much!

Can someone explain this to me? Transgenic Organism.? is a post from: Nature Technology Corporation

Technorati Tags: , , , , , , , , ,

]]> http://www.natx.com/topblog/can-someone-explain-this-to-me-transgenic-organism/feed 1 DNA strawberry extraction lab? http://www.natx.com/topblog/dna-strawberry-extraction-lab http://www.natx.com/topblog/dna-strawberry-extraction-lab#comments Wed, 24 Feb 2010 05:46:46 +0000 NTC http://www.natx.com/topblog/dna-strawberry-extraction-lab DNA strawberry extraction lab? is a post from: Nature Technology Corporation

]]> In the DNA strawberry extraction expirement what is the octopoidy and why does it help this experiment?

2. What do you think the DNA extraction buffer does?

3. what should pass through the test tube after straining it through the cheesecloth?
4. Would the DNA be the same in all the cells of the strawberry?
5. Give some reasons why scientist are studying the genetics of strawberries?

DNA strawberry extraction lab? is a post from: Nature Technology Corporation

Technorati Tags: , , , , , , , ,

]]> http://www.natx.com/topblog/dna-strawberry-extraction-lab/feed 2 what is the full name of CTEB which we use in DNA extraction?what is the use of adding that? http://www.natx.com/topblog/what-is-the-full-name-of-cteb-which-we-use-in-dna-extractionwhat-is-the-use-of-adding-that http://www.natx.com/topblog/what-is-the-full-name-of-cteb-which-we-use-in-dna-extractionwhat-is-the-use-of-adding-that#comments Tue, 23 Feb 2010 18:53:37 +0000 NTC http://www.natx.com/topblog/what-is-the-full-name-of-cteb-which-we-use-in-dna-extractionwhat-is-the-use-of-adding-that what is the full name of CTEB which we use in DNA extraction?what is the use of adding that? is a post from: Nature Technology Corporation

]]> and also…why we put Prokinase – K after putting the sample in the tube?After putting Pro-K , we will put the tube in the water bath around 60 degree celcius…and why we have to do that?is it for the Pro-K to digest all the sample(tissue)??I was trying to get DNA band for moth.any website can help me in DNA extraction?thanks ..

what is the full name of CTEB which we use in DNA extraction?what is the use of adding that? is a post from: Nature Technology Corporation

Technorati Tags: , , ,

]]> http://www.natx.com/topblog/what-is-the-full-name-of-cteb-which-we-use-in-dna-extractionwhat-is-the-use-of-adding-that/feed 1 what are sources of error for a DNA extraction lab? http://www.natx.com/topblog/what-are-sources-of-error-for-a-dna-extraction-lab http://www.natx.com/topblog/what-are-sources-of-error-for-a-dna-extraction-lab#comments Sat, 20 Feb 2010 00:17:20 +0000 NTC http://www.natx.com/topblog/what-are-sources-of-error-for-a-dna-extraction-lab what are sources of error for a DNA extraction lab? is a post from: Nature Technology Corporation

]]> DNA extraction from an onion…no human errors

what are sources of error for a DNA extraction lab? is a post from: Nature Technology Corporation

Technorati Tags: ,

]]> http://www.natx.com/topblog/what-are-sources-of-error-for-a-dna-extraction-lab/feed 1 Sources of DNA for extraction? http://www.natx.com/topblog/sources-of-dna-for-extraction http://www.natx.com/topblog/sources-of-dna-for-extraction#comments Wed, 17 Feb 2010 09:36:21 +0000 NTC http://www.natx.com/topblog/sources-of-dna-for-extraction Sources of DNA for extraction? is a post from: Nature Technology Corporation

]]> I recently did a DNA extraction using my own cheek cells, and I was wondering if there are any other sources of DNA that are better?

Is it better to use blood, hair cells, skin cells or semen for example?

Sources of DNA for extraction? is a post from: Nature Technology Corporation

Technorati Tags: , , , ,

]]> http://www.natx.com/topblog/sources-of-dna-for-extraction/feed 2 why is cold salty water added to dna extraction..PLEASEE HELP!! =)? http://www.natx.com/topblog/why-is-cold-salty-water-added-to-dna-extraction-pleasee-help http://www.natx.com/topblog/why-is-cold-salty-water-added-to-dna-extraction-pleasee-help#comments Sun, 14 Feb 2010 10:49:10 +0000 NTC http://www.natx.com/topblog/why-is-cold-salty-water-added-to-dna-extraction-pleasee-help why is cold salty water added to dna extraction..PLEASEE HELP!! =)? is a post from: Nature Technology Corporation

]]> can anyone pleaseee tell me (in sorta scientific terms) why cold salty water is added to the split pea mixture when doing a dna extraction..i cant find the answer anywhere! thanx

why is cold salty water added to dna extraction..PLEASEE HELP!! =)? is a post from: Nature Technology Corporation

Technorati Tags: , , ,

]]> http://www.natx.com/topblog/why-is-cold-salty-water-added-to-dna-extraction-pleasee-help/feed 2 can lemon be used as an enzyme for DNA extraction? http://www.natx.com/topblog/can-lemon-be-used-as-an-enzyme-for-dna-extraction http://www.natx.com/topblog/can-lemon-be-used-as-an-enzyme-for-dna-extraction#comments Fri, 12 Feb 2010 06:00:31 +0000 NTC http://www.natx.com/topblog/can-lemon-be-used-as-an-enzyme-for-dna-extraction can lemon be used as an enzyme for DNA extraction? is a post from: Nature Technology Corporation

]]> it’s an experiment. i dont have meat tenderizer, contact lens cleaner, or pineapple juice on hand. so i want to know if lemon juice can be used as an enzyme in DNA extraction. please answer ASAP

can lemon be used as an enzyme for DNA extraction? is a post from: Nature Technology Corporation

Technorati Tags: , , , , , ,

]]> http://www.natx.com/topblog/can-lemon-be-used-as-an-enzyme-for-dna-extraction/feed 2 Need help on DNA extraction activity? http://www.natx.com/topblog/need-help-on-dna-extraction-activity http://www.natx.com/topblog/need-help-on-dna-extraction-activity#comments Thu, 11 Feb 2010 18:43:34 +0000 NTC http://www.natx.com/topblog/need-help-on-dna-extraction-activity Need help on DNA extraction activity? is a post from: Nature Technology Corporation

]]> Im working on a DNA extraction activity and i need some help,,,it was done using onions.

1. Why were the onion cellse heated?

2. DNA was spooled out using a glass rod – how do you account for the "stickiness" of DNA to glass?

3. Why was the onion mashed? What would happen if you didnt do this step?

4. WHy was detergent added?

5. How does NaCl contribute to a maximum DNA extraction?

6. What is the purpose of adding cold ethanol?

Need help on DNA extraction activity? is a post from: Nature Technology Corporation

Technorati Tags: , , , , , ,

]]> http://www.natx.com/topblog/need-help-on-dna-extraction-activity/feed 1 Can blood sample which has been tested for erythrocyte sedminentation rate (ESR) be used for DNA extraction? http://www.natx.com/topblog/can-blood-sample-which-has-been-tested-for-erythrocyte-sedminentation-rate-esr-be-used-for-dna-extraction http://www.natx.com/topblog/can-blood-sample-which-has-been-tested-for-erythrocyte-sedminentation-rate-esr-be-used-for-dna-extraction#comments Thu, 11 Feb 2010 18:43:17 +0000 NTC http://www.natx.com/topblog/can-blood-sample-which-has-been-tested-for-erythrocyte-sedminentation-rate-esr-be-used-for-dna-extraction Can blood sample which has been tested for erythrocyte sedminentation rate (ESR) be used for DNA extraction? is a post from: Nature Technology Corporation

]]> Chemicals are added to dilute blood before it made to stand in the Westegren’s apparatus for an hour. Does it have any effetc on DNa extraction.

Can blood sample which has been tested for erythrocyte sedminentation rate (ESR) be used for DNA extraction? is a post from: Nature Technology Corporation

Technorati Tags: ,

]]> http://www.natx.com/topblog/can-blood-sample-which-has-been-tested-for-erythrocyte-sedminentation-rate-esr-be-used-for-dna-extraction/feed 3