Recombinant DNA Technology Question.. Please Help! Did I do it right?
A potential therapeutic hepatitis C treatment can be manufactured using E. coli to express one of the hepatitis C proteins via recombinant DNA technology. The hepatitis C protein is derived from the hepatitis C virus genome and is used to stimulate the patient’s own immune system to attack the virus without exposure to the intact virus. Explain how you can create a strain of E. coli that will produce the therapeutic protein using the following materials: plasmid, restriction enzyme, DNA ligase, hepatitis C virus DNA.
First, one has to use a restriction enzyme to cut the hepatitis C virus DNA from the hepatitis C virus genome and the plasmid of the E. coli. Second, one has to add the gene from the hepatitis C virus genome, which allows the creation of the hepatitis protein, to the plasmid of the E. coli, which has to also be cut by a restriction enzyme to allow the gene from the virusgenometobeaddedtotheE.coliplasmid. Last,onehastoaddDNAligasetotheequationtorepairthesugarphosphatebonds
2 Responses
max
06 Mar 2010
CONtentious
06 Mar 2010
Yup sounds right to me. You still have to put it in E. coli to express as Max said but, spot on answer. I have nothing to contribute lol.
Well I guess you could use electroporation instead of heat but eh whatever.
you have to heat up the E. coli so the pores open and the virus DNA can enter the bacteria