How does recombinant DNA containing Bt gene incorporate the gene into the genome of the organism?
i know how Ti plasmid may be used as a vector carrying the Bt gene into a maize cell, but how is the actual Bt gene incorporated into the genome of the corn plant, thus making it Bt corn?
One Response
archaeadoc
02 Mar 2010
So, I think you are asking about the mechanism for integration of the DNA on the Ti plasmid into the DNA of the corn plant.
That means that you know that there are proteins that coat and protect the introduced DNA from degradation. Once in the nucleus, as it is with mammalian cells, there is non-homologous recombination of DNA that leads to integration. Integrations occur at random positions, so the same gene introduced into a plant will produce different locations for the integration event.
Are you asking about the specifics of that mechanism? For that I would look at double strand break repair mechanisms and read on that topic. Here is a reference.