Following plasmid purification what impurities could be present in the blob I can see at the end of the gel?
I did a phenol chloroform extraction and an ethanol precipitation ( started with E coli culture containing my plasmid – was in YT broth).
The photo of my gel shows a blob at the end of the lane( furthest from well) what impurities would be in this and how is it best to remove them?
Many thanks
