Which of the following is NOT part of the recombinant DNA technology?

a. restriction enzymes

b. polymerase chain reaction (PCR)

c. glucose

d. plasmids

Plasmids are usually treated with alkaline phosphatase to prevent self-ligation.In such a case,can the inserted fragment be incoporated into plasmid in more than two orientations?

A. the growth of the plasmid DNA.

B. DNA replication of plasmids outside a host cell.

C. the process of growth and division of the host cell.

D. transformation of bacterial cells.

A scientist is using an ampicillin-sensiitive strain of bacteria that cannot use lactose because it has a nonfunctional gene in the lac operon. She has to plasmids. One contains a functional copy of the affected gene of the lac operon, and the other contains the gene for ampicillin resistance. Using restriction enzymes and DNA ligase, she forms a recombinant plasmid containing both genes. She then adds a high concentration of the plasmid to a tube off the bacteria in a medium for bacterial growth that contains glucose as thee only energy source. This tube (+) and a control tube (-) with similar bacteria but no plasmid are both incubated under the appropriate conditions for growth and plasmid uptake. The scientist then spreads a sample of each bacterial culture (+ and – ) on each of the three types of plates indicated below.

In a hypothetical situation, the genes for sex pilus construction and for tetracycline resistance are located together on the same plasmid within a particular bacterium. If this bacterium readily performs conjugation involving a copy of this plasmid, then the result should be..

A) the production of endospores among the bacterium’s progeny.
B) the rapid spread of tetracycline resistance to other bacteria in that habitat.
C) the temporary possession by this bacterium of a completely diploid genome.
D) a transformed bacterium
E) the subsequent loss of tetracycline resistance from this bacterium.

using E.coli containig plasmids DNA carrying gene for GH, DNA ligase a restriction enzyme and growth medium

a. recombinant plasmids of bacteria
b. recombinant viral DNA
c. eukaryotic chromosomes
d. A and B
E. A, B, and C

Include in your discussion the role played by plasmids, viruses, and restriction endonucleases. Name and describe five medical applications of genetically engineered products and give the organism used to produce that product.

Scientists developed a set of guidelines to address the safety of DNA technology. Which of the following is one of the adopted safety measures?

A)Microorganisms used in recombinant DNA experiments are genetically crippled to ensure that they cannot survive outside of the laboratory.
B)Genetically modified organisms cannot be part of our food supply.
C)Transgenic plants are engineered so that the plant genes cannot hybridize.
D)Experiments involving HIV or other potentially dangerous viruses have been banned.
E)Recombinant plasmids cannot be replicated.

B. are sites for inserting genes for amplification. C. may be transfered between different species of bacteria D. may confer the ability to donate genetic material when bacteria conjugate E. all of these