Just wondering what some differences between DNA and RNA are? What are the relative lengths of a DNA and RNA strand?
Also, why is RNA referred to also as mRNA?
What is the template/antisense strand of DNA, and the coding/sense strand?
Thanks!
10. Which one of the following statements about using reverse genetic techniques to produce disease models
in knockout mice is INCORRECT?
(A) The sequence of the gene must be known before the gene can be knocked out.
(B) The targeting vector contains a disrupted copy of the gene where all or part of that gene has been
replaced by a selectable marker.
(C) The targeting vector is transformed into embryonic stem (ES) cells, where it will recombine with the
ES cell DNA.
(D) The knockout mice are missing one good copy of the gene in all cells.
I am studying how DNA probes can detect harmful alleles. The book I am reading describes that an allele for something like cystic fibrosis can be detected in a heterozygous individual by its restriction site. I understand the concept, I am just curious how an enzyme can cut and paste an allele, and how it knows which one to focus on?
a. Vector
b. Reverse Transcriptase
c. Trasnport molecule
d. Genetic probe
e. PCR device
Foreign DNA is spliced into a plasmid vector. The recombined plasmid then carries the foreign DNA into the bacterial cell, where it replicates independently of the bacterial chromosome. is it True or False?
If the foreign DNA contained a gene for human growth hormone, each cell will make the hormone.
What is different about the DNA fragments created by digestion with Hpa I as compared to the DNA fragments created by digestion with Eco RI?
Are there any disadvantages of using adenoviruses instead of retroviruses as vectors in gene therapy
For example if a person died, how long post-mortem could a lab identify their DNA, and what would be the best cells to tell what the person looked like and their biological construction? I’m doing some science fiction writing and I’m hoping someone could help me with this research.
How could one change in a DNA nucleotide alter the formation of the translated protein? ( An example would be the difference between normal and sickle-cell hemoglobin.)
Could anyone give me a good site or advice of their own to help me understand better Cloning vectors and their main role, how they work… And Transplanting Genes and how insulin is involved?
How is recombinant DNA formed? Any helpful advice will be greatly appreciated!!!
