Nature Technology Corporation » Genes

Express protein in Bacteria.?

NTC — Wed, 28 Jul 2010 18:48:22 +0000

im working with AGO1 which required for leaf development. I express it in the bacteria. Why I needa do so? Just for amplification? Other than that, wot can the expression vector does? Will it help me to identify gene and its function? and how?

Express protein in Bacteria.? is a post from: Nature Technology Corporation

Technorati Tags: amplification, bacteria, expression vector, leaf development, wot

GENETICS/GENOMES please help i don’t understand it!!!?

NTC — Tue, 27 Jul 2010 18:48:30 +0000

you are interested in overproducing the protein product of a human gene (that has one intron in it) in a bacterial host (where no splicing of the intron from the mRNA can occur). To get a copy of the gene without the intron, you try to do cDNA cloning starting with poly(A)^+ mRNA. after several failed attempts to find the cDNA clone, you come to the conclusion that the mRNA is most likely very unstable. suggest an alternate approach to producing an intron-free gene, ready for cloning in an expression vector.

GENETICS/GENOMES please help i don’t understand it!!!? is a post from: Nature Technology Corporation

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genetics help!! please help i don’t understand it!!!?

NTC — Thu, 22 Jul 2010 17:49:14 +0000

genetics help!! please help i don’t understand it!!!? is a post from: Nature Technology Corporation

Technorati Tags: alternate approach, bacterial host, cdna clone, cloning, conclusion, expression vector, failed attempts, human gene, intron, mrna, protein product

How can you isolate and define a minimal E. coli promoter?

NTC — Wed, 21 Jul 2010 20:00:47 +0000

I have learned of a method by which scientists isolated an E.coli origin of replication and determined a minimal ori. It is done by making a genomic library by introducing different fragments of an E.coli into one E.coli and then seeing which one reproduces which would mean that it contained the origin. Then an enzyme was introduced, that chewed away at the gene from either size until it was determined what the origin size was

I need to modify it appropriately so that you can isolate and define a minimal E.coli promoter.

I am specifically interested in knowing about the selection scheme, modifications of the vector platform if necessary, any reporter gene assays etc – so please highlight these in your approach and provide me with a rationale for any alterations from the initial method. Thank you!
instead of lacz can you uase a temperature sensitive mutant?

How can you isolate and define a minimal E. coli promoter? is a post from: Nature Technology Corporation

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please help with this bio problem!!!?

NTC — Wed, 21 Jul 2010 18:12:25 +0000

ok i know i should do all my hw myself but i dont know where to even start!!! please help!!!!

1st one

your teamat the national institutes of health is working on a gene therapy treatment for the delta F508 mutation in a cystic fibrosis transmembrane conductance regulator gene. using an adeno-associated virus as the vector, describe the procedures your group will employ to prepare for and successfully deliver a healthy gene to cells of the affected tissue

k here is the next one

your hot shot biotech team attempts to straighten out a potential problem. some lab technicians might have accidentally mixed samples of DNA from several sources. Using the Southern Blot Technique, describe how you can identify the DNA fragments in the mixture that carry the PAH gene (a mutation in this gene, located on chromosome 12, can cause phenylketonuria)

im pretty sure for the second one you would compare a straight sample of PAH to the DNA samples

please help with this bio problem!!!? is a post from: Nature Technology Corporation

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Biology question? Please help?

NTC — Mon, 19 Jul 2010 18:12:55 +0000

1) Four of the five answers listed below are aspects of the process known as gene splicing, select the exception?
A) cloning vector like virus
B) restriction enzymes
C) sticky ends
D) exposed base pairs
E) crossing over

Biology question? Please help? is a post from: Nature Technology Corporation

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How did James Watson contribute to the discovery of the structure of DNA?

NTC — Sat, 17 Jul 2010 18:01:19 +0000

How did James Watson contribute to the discovery of the structure of DNA?

How did James Watson contribute to the discovery of the structure of DNA? is a post from: Nature Technology Corporation

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In the experiment completed by Hematech Inc. to knockout the prion protein in cattle, what was the…?

NTC — Thu, 15 Jul 2010 18:12:54 +0000

type of vector they used? Did they use a plasmid, gene gun, virus?

In the experiment completed by Hematech Inc. to knockout the prion protein in cattle, what was the…? is a post from: Nature Technology Corporation

Technorati Tags: gene gun, vector, virus

How many molecules of DNA are there in the nucleus?

NTC — Thu, 15 Jul 2010 17:36:28 +0000

Humans have like 24 chromosomes. Are chromosomes made out of separate strands of DNA or are they all connected to each other, like one VERY Very long molecule? I don’t get it.

How many molecules of DNA are there in the nucleus? is a post from: Nature Technology Corporation

Technorati Tags: chromosomes, dna, molecule, strands

How can there not be enough DNA at a crime scene to identify a criminal?

NTC — Wed, 14 Jul 2010 17:36:11 +0000

Ben Roethlisberger was just let off of allegations of sexual assault because they could not match him to the DNA found on the victim since there was not enough. In school, we learned that you can use the PCR to multiply DNA into millions of copies from 1 strand. How could there not be enough DNA to create a profile?

How can there not be enough DNA at a crime scene to identify a criminal? is a post from: Nature Technology Corporation

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