Why is it necessary for a plasmid vector that can be used for molecular cloning in bacteria to carry a
gene that can provide drug resistance to its host bacterium?

In a study done, a vector was inserted with a p element and the gene for white eyes, but since that’s a recessive allele, it must’ve interrupted the wildtype red allele… if this is true, how was it done?

Please explian in details ( or refer to a paper ,website etc) the steps required to clone a specific gene e.g (Colicin gene, a mobilizable plasmid like RSF1010) into a conjugative plasmid .
The Questions that I dont know about gene cloning in a plasmid are:

1. How do we make a restricition site into gene to be cloned? and do we alwayes need to do that ?
2. Does it matter if the gene cloned downstream or upstream of any other gene ; e.g ( ori gene or antibiotic marker gene … Etc..) or Should We clone in it in a specific site in order to be properly translated?
3. How do we detect the presence of the cloned gene after we do the cloning , I prefer to put another antibiotic marker but i dont know how exactly to do it

Thanks alot

"You are attempting to introduce a gene that imparts larval moth resistance to bean plants. Which of the following vectors are you most likely to use?"

a. phage DNA
b. E. coli Plasmid
c. Ti plasmid
d. yeast plasmid
e.bacterial artificial chromosome

my book stipulates nothing about these and I’m lost :[[

In the process of human gene cloning using recombinant plasmids, the bacterial plasmid
A. functions as a vector.
B. s the source of the gene to be cloned.
C. is cultured inside the human cell, which contains the gene to be cloned.
D. is used to insert the human gene into the bacterial chromosome.
E. None of the choices are correct.

1. describe the process of self-assembly as it pertains to viruses.

2. Describe one way that eukaryotic genes can be shut off.

Please everyone, please help me to answer these questions. I appreciate your help so much!
Thank you, thank you…

Which of the following is a primary participant in cloning an isolated gene?
a. restriction endonuclease
b. vector
c. host organism
d. all of these

Please use easy to understand words. I really hate biology. :[

i know how Ti plasmid may be used as a vector carrying the Bt gene into a maize cell, but how is the actual Bt gene incorporated into the genome of the corn plant, thus making it Bt corn?

Help! Please!