NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

NTC's Molecular Services
Linearized Cloning Vectors

NTC Products and Services

VECTORS
XMammalian Vectors
XAntibiotic-Free Vecotrs
XRig-I Activating Vectors
XpDNAVACCUltra Vector
XLinear Vectors
XRecombineering Vectors
XAutolytic E. coli Cell Lines
XGene Sllencing (transient)
XGene Sllencing (retroviral, stable)

XAnti-Silencing Elements

CELL LINES
XAutolytic E. coli Cell Lines

DNA VACCINES
X
RapidVACC™

RECOMBINANT PROTEINS
X
pVEX™
X
Fluorescent Proteins

PLASMIDS
X
Small Scale Plasmid DNA
X
Small Scale C of A
X
Large Scale Plasmid DNA
X
Large Scale C of A
X
Plasmid DNA Order Form
X
E. coli Cell paste
X
DNA FERMENTATION PROCESS
X
HyperGRO™ Process
X
HyperGRO™ Licensing
X
HyperGRO™ Pricing

PROCESS DEVELOPMENT
X
Process Development Basics
X
Process Licensing


HYPERTAQ™ DNA POLYMERASE

NTC Vector Development ServicesX|XE. coli Strain EngineeringX|XLinearized Cloning VectorsX|XRapid-VACC™ DNA vaccine cloningX|XBiologics Product DevelopmentX|XPlasmid Production


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1(888) WIz-BANG

Linearized Cloning Vectors
Cloning in plasmids is usually accomplished by linearizing the plasmid with restriction enzymes, dephosphorylation, and gel-isolation.  Ordinarily, this causes several problems that lead to decreased cloning efficiency.  First, dephosphorylation decreases cloning efficiency approximately 50 fold. Ethidium bromide gel stain then forms adducts and intercalates the DNA, creating mutations and decreasing cloning efficiency. Ultraviolet light (both long and short wave) then degrades the DNA.  Agarose inhibitors interfere with cloning efficiency.  Finally, ‘shadow-band’ extraction allows residual uncut pDNA to survive the entire process, resulting in background (parental) plasmids.

NTC eliminates all of these issues, providing you with high-efficiency cloning vectors that have a 4-5 log reduction of background while retaining the cloning efficiency of plasmids that have not been gel isolated. This is perfect for high throughput screening, library production, and is a useful service for biotech suppliers who wish to provide their customers with highest quality cloning vectors, ready to use.


Contact NTC, Toll free: 1-(888) WIZ-BANG

Plasmids, Recombinant Proteins, DNA Vaccines, Viral Vectors, Therapeutic Genes, Fermentation Technology for Gene Therapy and Biotechnology
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including Databases and Sequences Analysis Tool
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