2010 NTC Vector Prices:
Mammalian Expression Vectors/DNA Vaccine Vectors
Kanamycin-Resistance Vectors
Plasmid Expression Vectors
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC7482
|
secreted
|
20 µg
|
NTC- DV7482
|
$320.00
|
|
NTC7485-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC- DV7485-EGFP
|
$320.00
|
* The NTC7485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC7485 backbone for cloning new genes
Linearized Vectors‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC7482
|
secreted
|
1 µg
|
NTC- DV7482-LV
|
$344.00
|
|
NTC7485
|
native
|
1 µg
|
NTC- DV7485-LV
|
$344.00
|
‡ SalI - BglII linearized vector sufficient for 20 cloning reactions
RAPID-VACC™
RAPID-VACC™ CONTRACT CLONING is an optional service whereby NTC clones investigator-specified genes into the desired expression vectors, providing sequence-validated clones, or purified plasmid DNA ready for animal testing (see www.natx.com ).
Seamless cloning gene of interest into any vector
|
$400.00
|
1mg endotoxin-free plasmid preparations, each
|
$335.00*
|
* For larger quantities of plasmid DNA, see:
NTC Plasmid page.
NTC offers the following products for use cloning with the NTC7482 and NTC7485 vectors
Accessory Products
|
Product
|
Description
|
Quantity
|
Catalog Number
|
Price
|
|
pVAX5’
|
Forward sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP1
|
$30.00
|
|
pVAX3’
|
Reverse
sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP2
|
$30.00
|
Antibiotic-Free (RNA-OUT) Vectors
Plasmid Expression Vector kit‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC8482
|
secreted
|
20 µg
|
NTC- DV8482
|
$420.00
|
|
NTC8485-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC- DV8485-EGFP
|
$420.00
|
* The NTC8485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC8485 backbone for cloning new genes
‡ Kit contains plasmid and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
Linearized Vector kit‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC8482
|
secreted
|
1 µg
|
NTC- DV8482-LV
|
$444.00
|
|
NTC8485
|
native
|
1 µg
|
NTC- DV8485-LV
|
$444.00
|
‡ SalI - BglII linearized vector sufficient for 20 cloning reactions and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
RAPID-VACC™ CONTRACT CLONING is an optional service whereby NTC clones investigator-specified genes into the desired expression vectors, providing sequence-validated clones, or purified plasmid DNA ready for animal testing (see www.natx.com ).
RAPID-VACC™
| Seamless cloning gene of interest into any vector |
$400.00 |
| 1mg endotoxin-free plasmid preparations, each |
$335.00* |
NTC offers the following products for use the NTC8482 and NTC8485 vectors
Accessory Products
|
Product
|
Description
|
Quantity
|
Catalog Number
|
Price
|
|
pVAX5’
|
Forward sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP1
|
$30.00
|
|
pVAX3’
|
Reverse
sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP2
|
$30.00
|
|
DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR electrocompetent cells
|
Host strain for NTC8482 and NTC8485 selection
|
0.5 mL
|
NTC-DVU-CC1
|
$199.00
|
|
Sucrose selection agar
|
Plates for NTC8482 and NTC8485 selection
|
Pouch to make 1 L agar*
|
NTC-DVU-MD1
|
$20
|
|
Sucrose selection media
|
Media for NTC8482 and NTC8485 culture
|
Pouch to make 1 L media*
|
NTC-DVU-MD2
|
$20
|
* Reconstitute with distilled water to 1L and autoclave prior to use
Plasmid Expression Vectors (parent vectors without eRNA41H)
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC7482
|
secreted
|
20 µg
|
NTC- DV7482
|
$320.00
|
|
NTC7485-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC- DV7485-EGFP
|
$320.00
|
* The NTC7485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC7485 backbone for cloning new genes
Plasmid Expression Vector kit (parent vectors without eRNA41H)‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC8482
|
secreted
|
20 µg
|
NTC- DV8482
|
$420.00
|
|
NTC8485-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC- DV8485-EGFP
|
$420.00
|
* The NTC8485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC8485 backbone for cloning new genes
‡ Kit contains plasmid and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
Linearized Vectors (parent vectors without eRNA41H)‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC7482
|
secreted
|
1 µg
|
NTC- DV7482-LV
|
$344.00
|
|
NTC7485
|
native
|
1 µg
|
NTC- DV7485-LV
|
$344.00
|
‡ SalI - BglII linearized vector sufficient for 20 cloning reactions
Linearized Vector kit (parent vectors without eRNA41H)‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC8482
|
secreted
|
1 µg
|
NTC- DV8482-LV
|
$444.00
|
|
NTC8485
|
native
|
1 µg
|
NTC- DV8485-LV
|
$444.00
|
‡ SalI - BglII linearized vector sufficient for 20 cloning reactions and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
Accessory Products
|
Product
|
Description
|
Quantity
|
Catalog Number
|
Price
|
|
pVAX5’
|
Forward sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP1
|
$30.00
|
|
pVAX3’
|
Reverse
sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP2
|
$30.00
|
|
DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR electrocompetent cells
|
Host strain for NTC8482 and NTC8485 selection
|
0.5 mL
|
NTC-DVU-CC1
|
$199.00
|
|
Sucrose selection agar
|
Plates for NTC8482 and NTC8485 selection
|
Pouch to make 1 L agar*
|
NTC-DVU-MD1
|
$20
|
|
Sucrose selection media
|
Media for NTC8482 and NTC8485 culture
|
Pouch to make 1 L media*
|
NTC-DVU-MD2
|
$20
|
* Reconstitute with distilled water to 1L and autoclave prior to use
RIG-1-activating DNA vaccine plasmids and linear cloning vectors:
Kanamycin Selection Plasmid Expression Vectors
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC7482-eRNA41H
|
secreted
|
20 µg
|
NTC- DV7482-41H
|
$320.00
|
|
NTC7485-eRNA41H-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC- DV7485-41H-EGFP
|
$320.00
|
* The NTC7485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC7485 backbone for cloning new genes
Kanamycin Selection Linearized Vectors‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC7482-eRNA41H
|
secreted
|
1 µg
|
NTC- DV7482-41HLV
|
$344.00
|
|
NTC7485-eRNA41H
|
native
|
1 µg
|
NTC- DV7485-41HLV
|
$344.00
|
‡ SalI - BglII linearized vector sufficient for 20 cloning reactions
Sucrose Selection Plasmid Expression Vector kit‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC8482-eRNA41H
|
secreted
|
20 µg
|
NTC- DV8482-41H
|
$420.00
|
|
NTC8485-eRNA41H-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC- DV8485-41H-EGFP
|
$420.00
|
* The NTC8485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC8485 backbone for cloning new genes
‡ Kit contains plasmid and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
Sucrose selection Linearized Vector kit‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
NTC8482 eRNA41H
|
secreted
|
1 µg
|
NTC- DV8482-41HLV
|
$444.00
|
|
NTC8485 eRNA41H
|
native
|
1 µg
|
NTC- DV8485-41HLV
|
$444.00
|
‡ SalI - BglII linearized vector sufficient for 20 cloning reactions and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
pDNAVACCUltra DNA VaccineVectors for Genetic Immunization
Plasmid Vectors
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
pDNAVACCultra1
|
secreted-endosome
|
20 µg
|
NTC-DVU1
|
$299.00
|
|
pDNAVACCultra2
|
secreted
|
20 µg
|
NTC-DVU2
|
$299.00
|
|
pDNAVACCultra3
|
secreted –GPI membrane-anchored
|
20 µg
|
NTC-DVU3
|
$299.00
|
|
pDNAVACCultra4
|
proteasome
|
20 µg
|
NTC-DVU4
|
$299.00
|
|
pDNAVACCultra5
|
native
|
20 µg
|
NTC-DVU5
|
$299.00
|
|
pDNAVACCultra6
|
GPI membrane-anchored
|
20 µg
|
NTC-DVU6
|
$299.00
|
|
pDNAVACCultra7
|
endosome
|
20 µg
|
NTC-DVU7
|
$299.00
|
|
pDNAVACCultra5-EGFP*
|
cytoplasmic EGFP
|
20 µg
|
NTC-DVU5-EGFP
|
$299.00
|
|
pDNAVACCultra kit (vectors 1- 5)
|
All destinations kit‡
|
20 µg each
|
NTC-DVU1-5
|
$1,196.00
|
|
pDNAVACCultra kit (vectors 4-7)
|
All destinations kit‡
|
20 µg each
|
NTC-DVU4-7
|
$957.00
|
* The pDNAVACCultra5-EGFP control plasmid is available for use as a transfection control for expression in a cell line of interest
‡ Vector kit 1-5 allows targeting of an antigen to secreted, endosomal, membrane-anchored, proteosome, or native destinations using, where necessary, a vector encoded TPA secretion sequence (pDNAVACCultra1-3). Vector kit 4-7 allows targeting of an antigen to secreted, endosomal, membrane-anchored, or proteosome destinations using, where necessary, an investigator specified secretion sequence (e.g. IgK ).
Recombineering Vectors
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
pKD46-recA
|
Escherichia coli
|
20 µg
|
NTC-RV-RecA
|
$320.00
|
|
pKD46-recApa
|
Pseudomonas aeruginosa
|
20 µg
|
NTC-RV-RecApa
|
$320.00
|
Linearized Vectors‡
|
Vector
|
Targeting
|
Quantity
|
Catalog Number
|
Price
|
|
pDNAVACCultra1
|
secreted-endosome
|
1 µg
|
NTC-DVU1-LV
|
$344.00
|
|
pDNAVACCultra2
|
secreted
|
1 µg
|
NTC-DVU2-LV
|
$344.00
|
|
pDNAVACCultra3
|
secreted –GPI membrane-anchored
|
1 µg
|
NTC-DVU3-LV
|
$344.00
|
|
pDNAVACCultra4
|
proteasome
|
1 µg
|
NTC-DVU4-LV
|
$344.00
|
|
pDNAVACCultra5
|
native
|
1 µg
|
NTC-DVU5-LV
|
$344.00
|
|
pDNAVACCultra6
|
GPI membrane-anchored
|
1 µg
|
NTC-DVU6-LV
|
$344.00
|
|
pDNAVACCultra7
|
endosome
|
1 µg
|
NTC-DVU7-LV
|
$344.00
|
|
pDNAVACCultra kit (vectors 1- 5)
|
All destinations kit
|
1 µg each
|
NTC-DVU1-5-LV
|
$1,376.00
|
|
pDNAVACCultra kit (vectors 4-7)
|
All destinations kit
|
1 µg each
|
NTC-DVU4-7-LV
|
$1099.00
|
‡ SapI linearized vector sufficient for 20 cloning reactions
NTC offers the following products for use cloning with the pDNAVACCUltra™ vectors
Accessory Products
|
Product
|
Description
|
Quantity
|
Catalog Number
|
Price
|
|
pVAX5’
|
Forward sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP1
|
$30.00
|
|
pVAX3’
|
Reverse
sequencing primer
|
500 pmol
(10 pmol/µL)
|
NTC-DVU-SP2
|
$30.00
|
|
