NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

Recombinant Proteins
Products, Vectors, Services and Prices


NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

pVEX™ Protein Expression Vectors
Catalogue No.
Marker
Description
Cloning
 Pricing
pVEX
NTC-PV-AR
AmpR
N-term-HN peptide tag
SapI cloning
 $299.00
pVEX HN
NTC-PV-ARHN
AmpR
N-terminal metal binding HN peptide tag
SapI cloning
 $299.00
pVEXK
NTC-PV-KR
KanR
N-term-HN peptide tag
SapI cloning
 $299.00
pVEXK HN
NTC-PV-KRHN
KanR
N-terminal metal binding HN peptide tag
SapI cloning
 $299.00
pVEXK HN-MCS-K6EE
NTC-PV-KRHNK6
KanR
C-term stability tag
Polylinker cloning
 $299.00
pVEXK HN-pfMBP-MCS-K6EE
NTC-PV-KRHNTS
KanR
N terminal dual function tag (metal binding HN peptide tag-thermostable pfMBP tag) - C terminal stability tag
Polylinker cloning
 $299.00
pVEXK pR pL HN-MBP-MCS-K6EE
NTC-PV-KRHNTS
KanR
N terminal dual function tag (metal binding HN peptide tag-thermostable pfMBP tag) - C terminal stability tag
Polylinker cloning
 $299.00
pVEXK pR pL HN-MCS-K6EE
NTC-PV-KRHNK6
KanR
N terminal metal binding HN peptide tag - C terminal stability tag
Polylinker cloning
 $299.00

Vector Services
NTC-PV-CLON pVEX cloning service for protein expression
$400.00
NTC-PV-CEX pVEX-cloned vector protein expression (solubility + yield determination)
$150.00
Protein Thermostability Engineering Service:

Clone gene of interest into pfMBP vector (customer defined, either pVEXK HN-MBP-MCS-K6EE or
pVEXK pR pL HN-MBP-MCS-K6EE), screen for passenger protein thermostabilization in small scale ($550).
Protein Production Services

• E. coli defined media fermentation cell paste

• Fermentation process development

NTC-PROD-PMDFRM Process Development, Plasmid, Fermentation
$3,200.00
NTC-PROD-PMDPUR Process Development, Plasmid, Purification
Inquire
NTC-PROD-PTNFRM Process Development, Protein, Fermentation
$3,600.00
NTC-PROD-PTNPUR Process Development, Protein, Purification
Inquire
IMAC purification services (for genes cloned into HN tagged vectors Nos.: 2, 4-8 with small scale protein solubility testing
= $550 upfront). Native or denaturing purification. ($250, 2 mg scale) Larger scale Inquire (based on yield from small
scale solubility testing)
Purification process development:  Pricing based on results of small scale yield and solubility testing ($550 upfront).
Specialty Services
• Vector retrofit to different promoter (existing = heat and IPTG inducible) ($500)

• Vector retrofit to different selection marker (existing = kanR) ($500)
NTC-VRFPRO Vector promoter retrofit service
$500.00
NTC-VRFPRO Vector promoter retrofit service $500.00