NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

Recombinant Proteins
pVEX™, Escherchia coli Expression Vectors

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

Escherchia coil Expression Vectors - pVEX™X|XFluorescent ProteinsX|XProducts, Vectors and ServicesX|XCofA (SAMPLE)gg


NTC pVEX™ Advantages:

• Highest yield recombinant Protein Production (up to 20g/L)
• Vaccine Antigen Production
• Native or Tagged Proteins
• Thermostable Tag (TST)
XXX-Thermostable Antigen
XXX-Thermostable Adjuvant
• C-tag Stability/Solubility
• Broad Increased Temperature IPTG Inducible Promoter
• Defined Media Fermentation
• Fluorescent Protein Production (Correct Folding)
• KanR or AmpR Selection
• Simultaneous Cloning
• High Throughput Type IIS Restriction Enzyme Cloning

 Flourescent Proteins, pVEX™, LacIQ repressor, inducible Tac promoter, optimized mRNA leader, HN affinity tag, thermostable antigen/adjuvant (MBP), and C-terminal tag for increased stability/solubility.

XXpVEXK HN-MBP-MCS-K6EE vector

Fluorescent Protein, pVEXK HN-MBP-MCS-K6EE

A typical advanced NTC pVEX™ protein expression vector, pVEXK HN-MBP-MCS-K6EE, with the following features: LacIQ repressor, inducible Tac promoter, optimized mRNA leader, HN affinity tag, thermostable antigen/adjuvant (MBP), and C-terminal tag for increased stability/solubility.
XXpVEX™ application notes:


Thermostable antigen and thermostable adjuvant: flagellin, a TLR5 agonist, was fused to thermostable maltodextrin binding protein (MBP) from Pyrococcus furiosus (TST-flagellin).
Thermostable antigen and thermostable adjuvant: flagellin, a TLR5 agonist, was fused to thermostable maltodextrin binding protein (MBP) from Pyrococcus furiosus (TST-flagellin).

Gel = NTC developed TLR5 receptor agonist TST-Flagellin. lane 1 = uninduced total protein, lane 2 = induced total protein, lane 3 = uninduced soluble protein, lane 4 = induced soluble protein, lane 5 = induced total protein (large scale fermentation harvest), lane 6 = induced soluble protein (large scale fermentation harvest), lane 7 = induced soluble protein after 75ºC 40 min heat treatment (large scale fermentation harvest), M= Marker