NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

Plasmid DNA Purification and Processing (PDF Version)
High-quality, fast turn-around plasmid DNA purification and production services

NTC Products and Services

VECTORS
XMammalian Vectors
XAntibiotic-Free Vecotrs
XRig-I Activating Vectors
XpDNAVACCUltra Vector
XLinear Vectors
XRecombineering Vectors
XAutolytic E. coli Cell Lines
XGene Sllencing (transient)
XGene Sllencing (retroviral, stable)

XAnti-Silencing Elements

CELL LINES
XAutolytic E. coli Cell Lines

DNA VACCINES
X
Prescion Cloning

RECOMBINANT PROTEINS
X
pVEX™
X
Fluorescent Proteins

PLASMIDS
X
Small Scale Plasmid DNA
X
Small Scale C of A (PDF)
X
Large Scale Plasmid DNA
X
Large Scale C of A (PDF)
X
Plasmid DNA Order Form (PDF)
X
E. coli Cell paste
X
Plasmid Detox Vector, NEW!
XX
DNA FERMENTATION PROCESS
X
HyperGRO™ Process
X
HyperGRO™ Licensing
X
HyperGRO™ Pricing

PROCESS DEVELOPMENT
X
Process Development Basics
X
Process Licensing


HYPERTAQ™ DNA POLYMERASE


NTC is an industry leading provider of highly-purified plasmids. Ordering plasmids from NTC is a fast and easy 2 step process, and there is no where else you will receive the attention to detail and great service for the cost.
NTC's plamid purification and processing includes:

• Individual constructs for researchers

• Large scale manufacturing for biotech suppliers and pharmaceutical companies.


Free of cesium chloride, ethidium bromide, and organic solvents

Analyzed by spectrophotometry for concentration and purity

Certificate of Analysis included

• Restriction enzyme analysis ensures the integrity of every order

• Transformation of DNA included

• Endotoxin levels < 0.1EU/mcg

• Additional Testing upon request, (see chart below)

At no additional charge, each endotoxin-free DNA prep includes:

1)
Transformation
2) Glycerol stock archiving
3)Lot sample archive
4) Custom formulation
5) Certificate of Analysis (C of A)

2 Steps to Ordering:

Timeline: First time orders require 2-3 weeks. Reorders can be from 1 day to two weeks, depending upon the arrangements made (just in time re-ordering is by prior agreement).

1) Call NTC for a quotation, (402) WIZ-BANG). Best pricing is available based on quantity ordered and copy number. Most high-copy plasmids have a pUC origin of replication, or equivalent, and result in the lowest cost. NTC is a longtime industry leader in quality and cost-effectiveness.

2) Send NTC 1mcg of your plasmid DNA, spotted on Whatman 3MM or FTE filter paper, together with NTC's Plasmid DNA Order Form filled out. (The form is designed to prompt you for all information needed for propagation and diagnostic testing.)


Plasmid Small / Large DNA Order Form

XXAAdditional services included with your plasmid prep:

• Transform the production host.

• Prepare a glycerol stock using "low metabolic burden technology"

• Grow, and purify the desired quantity of pDNA, using endotoxin-free reagents and procedures. 

• At no additional cost, NTC will also formulate the DNA according to specification (default is 0.5mg/ml in TE buffer). 

• A certificate of analysis will be provided including gel photo (digested and undigested DNA, ladder), concentration and quantity. The large-scale certificate of analysis will be provided, including: gel photo (digested and undigested DNA, ladder); concentration and quantity; information and testing for residual: RNA, genomic DNA (qPCR).

Additional testing for protein, endotoxin [LAL assay] and bioburden are available, upon request. (see table below) X

• In addition, NTC will retain an archival lot sample, and a glycerol stock of your plasmid for future reorders (at least one year retention). 

XXNTC's testing at no additional charge and cost comparison with NTC's competitorsX|XAdditional testing



XXAdditional testing is available upon request

Additional testing
Small Scale
Large Scale

Residual Protein: (modification BCA)

$75.00
$75.00

Residual RNA (spiked gel): (Small scale Only)

$75.00
-

Genomic DNA (qPCR): (Small scale Only)

$150.00
-

Endotoxin Testing: (Small scale Only)

$50.00
-

Bioburden

$50.00
$50.00

XXRelated molecular services, offered exclusively to industry partners:


Plasmids, Recombinant Proteins, DNA Vaccines, Viral Vectors, Therapeutic Genes, Fermentation Technology for Gene Therapy and Biotechnology
NTC's free resource "Site" for the biotechnology industry,
including Databases and Sequences Analysis Tool
s



WWW http://www.natx.com