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Plasmid DNA Purification and Processing (PDF Version)
High-quality, fast turn-around plasmid DNA purification and production services
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NTC is an industry leading provider of highly-purified plasmids. Ordering plasmids from NTC is a fast and easy 2 step process, and there is no where else you will receive the attention to detail and great service for the cost.
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NTC's plamid purification and processing includes:
• Individual constructs for researchers
• Large scale manufacturing for biotech suppliers and pharmaceutical companies.
• Free of cesium chloride, ethidium bromide, and organic solvents
• Analyzed by spectrophotometry for concentration and purity
• Certificate of Analysis included
• Restriction enzyme analysis ensures the integrity of every order
• Transformation of DNA included
• Endotoxin levels < 0.1EU/mcg
• Additional Testing upon request, (see chart below)
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At no additional charge, each endotoxin-free DNA prep includes:
1) Transformation
2) Glycerol stock archiving
3)Lot sample archive
4) Custom formulation
5) Certificate of Analysis (C of A)
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2 Steps to Ordering:
Timeline: First time orders require 2-3 weeks. Reorders can be from 1 day to two weeks, depending upon the arrangements made (just in time re-ordering is by prior agreement).
1) Call NTC for a quotation, (402) WIZ-BANG). Best pricing is available based on quantity ordered and copy number. Most high-copy plasmids have a pUC origin of replication, or equivalent, and result in the lowest cost. NTC is a longtime industry leader in quality and cost-effectiveness.
2) Send NTC 1mcg of your plasmid DNA, spotted on Whatman 3MM or FTE filter paper, together with NTC's Plasmid DNA Order Form filled out. (The form is designed to prompt you for all information needed for propagation and diagnostic testing.)
Plasmid Small / Large DNA Order Form
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| XXAAdditional services included with your plasmid prep: |
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• Transform the production host.
• Prepare a glycerol stock using "low metabolic burden technology"
• Grow, and purify the desired quantity of pDNA, using endotoxin-free reagents and procedures.
• At no additional cost, NTC will also formulate the DNA according to specification (default is 0.5mg/ml in TE buffer).
• A certificate of analysis will be provided including gel photo (digested and undigested DNA, ladder), concentration and quantity. The large-scale certificate of analysis will be provided, including: gel photo (digested and undigested DNA, ladder); concentration and quantity; information and testing for residual: RNA, genomic DNA (qPCR).
• Additional testing for protein, endotoxin [LAL assay] and bioburden are available, upon request. (see table below) X
• In addition, NTC will retain an archival lot sample, and a glycerol stock of your plasmid for future reorders (at least one year retention).
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XXNTC's testing at no additional charge and cost comparison with NTC's competitorsX|XAdditional testing
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| XXAdditional testing is available upon request |
| Additional testing |
Small Scale
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Large Scale
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Residual Protein: (modification BCA)
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$75.00
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$75.00
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Residual RNA (spiked gel): (Small scale Only)
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$75.00
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$150.00
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$50.00
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Bioburden
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$50.00
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$50.00
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| XXRelated molecular services, offered exclusively to industry partners: |
Contact:
Dr. Clague Hodgson
1 (888) WIZ-BANG (949-2264)
Email: hodgson@natx.com
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