NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

Mammalian Expression Vectors
Enhanced recombinant protein expression in mammalian cells and tissues: superior genetic immunization

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing
X• Vector Selection Flowchart

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

NTC Vector Advantages

• DNA Vaccination

• Therapeutic Vector

• Native Expression

• Secreted (TPA tag)

• Minimal Backbone

• Reporter (EGFP)

• Regulatory Compliance

• Simultaneous Cloning


Plus, choose from the following special advantages included on the Vector Selection Flowchart below.
A typical advanced NTC vector, NTC8685-EGFP, with the following features: markerless (sucrose selection), enhanced transcription (SV40/CMV), enhanced translation (CMV/HTLV-1-R), enhanced translation (VA1), enhanced plasmid production (PAS-BH).
A typical advanced NTC vector, NTC8685-EGFP, with the following features: markerless (sucrose selection), enhanced transcription (SV40/CMV), enhanced translation (CMV/HTLV-1-R), enhanced translation (VA1), enhanced plasmid production (PAS-BH).

XXVector Selection Flowchart

Selection
Transgene
Targeting
HTLV-I R
transient
expression
enhancer
VA1 transient
expression
enhancer
SV40-PAS-BH
increased
plasmid yield
backbone
RIG-I
activating
backbone
Vector
XKan XSecreted;
XGPI anchored;
XProteosome;
XEndosome;
Xor Native
No
No
No
No
 XpDNAVACCUltra
X Sucrose
XSecreted; or XNative
Yes
No
No
No
 XNTC8382
XNTC8385
XSucrose XSecreted; or XNative
Yes
Yes
No
No
 XNTC8382-VA1
XNTC8385-VA1
XKan XSecreted; or XNative
Yes
No
*Yes
No
 XNTC7482
XNTC7485
XKan XSecreted; or XNative
Yes
Yes
*Yes
Yes
 XNTC7482-eRNA41H
XNTC7485-eRNA41H
XSucrose
XSecreted; or XNative
Yes
No
 *Yes No XNTC8482
XNTC8485
XSucrose XSecreted; or XNative Yes Yes *Yes Yes XNTC8481-eRNA41H
XNTC8482-eRNA41H
XSucrose XSecreted; or XNative
Yes
Yes
*Yes
No
 XNTC8681
XNTC8682
XNTC8684
XNTC8685
XSucrose XSecreted; or XNative Yes Yes *Yes Yes XNTC8681-eRNA41H
XNTC8682-eRNA41H
XNTC8684-eRNA41H
XNTC8685-eRNA41H

*Save 20% off large scale production cost with NTC’s high-yield, SV40-PAS-BH vectors!
(for a limited time)
XXFigures 1-2


Figure 1: EGFP transgene expression (fluorescence) in human HEK293 cell line after transfection
of dcm+ or dcm- NTC8685, gWIZ and pVAX1 CMV promoter plasmid vectors.

EGFP transgene expression (fluorescence) in human HEK293 cell line after transfection of dcm+ or dcm- NTC8685, gWIZ and pVAX1 CMV promoter plasmid vectors.


Figure 2: NTC8385-VA1 expression compared to gWIZ-EGFP and pVAX1-EGFP in HEK293 cells.

Figure 2: NTC8385-VA1 expression compared to gWIZ-EGFP and pVAX1-EGFP in HEK293 cells.

XXVector List