NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

siLENTC™, NTC's Gene Silencing Vectors
siRNA Retroviral Vectors for Stable (Integrated) Expression of RNAI

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

siRNA Retroviral Vectors for Stable (Integrated) Expression of RNAI

XXTable 2:  U6 expression in various vectorsa

U6 expression in various vectorsa
a Vectors express a 100 bp ssRNA (eRNA18) for RT-PCR quantification 24 hr post tranfection (duplicate 24 wells, 2x tranfection = 4 wells per experiment); b pg eRNA18 target/100 ng total RNA isolated 24 hr post transfection; c Standardized to pGEM3F(+) U6 vector size  by multiplying pg RNA/7.2 pg by vector size in kb/3.6 kb; d Dimer contains two copies of eRNA18 per plasmid. This accounts for double the expression level


XXPlasmid-based (NTC) and retroviral (MLV) RNA interference vectors

shRNA Expression Vectors (RNA Interference) Catalogue Number
Retail Price
NTC7485E-U6-shRNA vector, KanR, 20 µg NTC-RI7485E-U6
$299.00
NTC8485E-U6-shRNA vector, antibiotic free, 20 µg NTC-RI8485E-U6
$299.00
NTC8485-U6-shRNA vector, antibiotic free, 20 µg NTC-RI8485-U6
$299.00
Vector, KanR, 20µg NTC-RIPMLV-U6
$299.00
pMLV GFP-neo H1 vector, KanR, 20 µg NTC-RIPMLV-H1
$299.00
NTC7485E-U6-shRNA linearized vector, gel isolated, KanR, 1 µg NTC-RI7485E-U6LV
$344.00
NTC8485E-U6-shRNA linearized vector, gel isolated, antibiotic free, 1 µg NTC-RI8485E-U6LV
$344.00
NTC8485-U6-shRNA linearized vector, gel isolated, antibiotic free, 1 µg NTC-RI8485-U6LV
$344.00
pMLV GFP-neo U6 linearized vector, gel isolated, KanR, 1 µg NTC-RIPMLV-U6LV
$344.00
pMLV GFP-neo H1 linearized vector, gel isolated, KanR, 1 µg NTC-RIPMLV-H1LV
$344.00
pRIP U6 5' forward sequencing primer, 500 pmol (10 pmol/μL) NTC-DVU-RI1
$30.00
pRIP H1 5' forward sequencing primer, 500 pmol (10 pmol/μL) NTC-DVU-RI2
$30.00
shRNA cloning service; customer-defined shRNA into vector and sequence verification NTC-RI-CLON
$400.00
Vector dimerization service NTC-VDIM
$400.00
Vector promoter retrofit service NTC-VRFPRO
$500.00
NTC4862 DH5 alpha host strain for 8-series vectors selection, 0.5 mL NTC-DVU-CC1
$199.00
NTC48165 DH5 alpha dcm- host strain for 8-series vectors selection, 0.5 mL NTC-DVU-CC2
$199.00
NTC48107 DH5 alpha dcm- host strain for 7-series vectors, 0.5 mL NTC-DVU-CC3
$199.00
NTC54207 XL1-Blue dcm- host strain for 7-series vectors, 0.5 mL NTC-DVU-CC4
$199.00
NTC54208 XL1-Blue dcm- host strain for 8-series vectors selection, 0.5 mL NTC-DVU-CC5
$199.00
NTC5402 XL1-Blue host strain for 8-series vectors selection, 0.5 mL NTC-DVU-CC6
$199.00
Sucrose selection agar for 8-series vectors selection, makes 1L agar NTC-DVU-MD1
$20.00
Sucrose selection media for 8-series vectors culture, makes 1L media NTC-DVU-MD2
$20.00