NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

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VECTORS | CELL LINES | VACCINES & ADJUVANTS | CLONING | PROTEINS | PLASMIDS | PROCESS DEVELOPMENT | DNA FERMENTATION PROCESS | HYPERTAQ | CONTRACT QC

Vectors
2010 NTC Vaccine/Vector Pricing

NTC Contacts:

Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

VectorsX|XServicesX|XPricingX|XManualsX|XPay Invoices Online

pDNAVACCUltra™ DNA Vaccine Vectors for Genetic Immunization
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
pDNAVACCultra1	secreted-endosome	20 µg	NTC-DVU1	$299.00
pDNAVACCultra2	secreted	20 µg	NTC-DVU2	$299.00
pDNAVACCultra3	secreted –GPI membrane-anchored	20 µg	NTC-DVU3	$299.00
pDNAVACCultra4	proteasome	20 µg	NTC-DVU4	$299.00
pDNAVACCultra5	native	20 µg	NTC-DVU5	$299.00
pDNAVACCultra6	GPI membrane-anchored	20 µg	NTC-DVU6	$299.00
pDNAVACCultra7	endosome	20 µg	NTC-DVU7	$299.00
pDNAVACCultra5-EGFP*	cytoplasmic EGFP	20 µg	NTC-DVU5-EGFP	$299.00
pDNAVACCultra kit (vectors 1- 5)	All destinations kit‡	20 µg each	NTC-DVU1-5	$1,196.00
pDNAVACCultra kit (vectors 4-7)	All destinations kit‡	20 µg each	NTC-DVU4-7	$957.00
*The pDNAVACCultra5-EGFP control plasmid is available for use as a transfection control for expression in a cell line of interest ‡Vector kit 1-5 allows targeting of an antigen to secreted, endosomal, membrane-anchored, proteosome, or native destinations using, where necessary, a vector encoded TPA secretion sequence (pDNAVACCultra1-3). Vector kit 4-7 allows targeting of an antigen to secreted, endosomal, membrane-anchored, or proteosome destinations using, where necessary, an investigator specified secretion sequence (e.g. IgK).
Linearized pDNAVACCultra™ Vectors‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
pDNAVACCultra1	secreted-endosome	1 µg	NTC-DVU1	$344.00
pDNAVACCultra2	secreted	1 µg	NTC-DVU2	$344.00
pDNAVACCultra3	secreted –GPI membrane- anchored	1 µg	NTC-DVU3	$344.00
pDNAVACCultra4	proteasome	1 µg	NTC-DVU4	$34400
pDNAVACCultra5	native	1 µg	NTC-DVU5	$344.00
pDNAVACCultra6	GPI membrane-anchored	1 µg	NTC-DVU6	$344.00
pDNAVACCultra7	endosome	1 µg	NTC-DVU7	$344.00
pDNAVACCultra5-EGFP*	cytoplasmic EGFP	1 µg	NTC-DVU5-EGFP	$344.00
pDNAVACCultra kit (vectors 1- 5)	All destinations kit‡	1 µg each	NTC-DVU1-5	$1,376.00
pDNAVACCultra kit (vectors 4-7)	All destinations kit‡	1 µg each	NTC-DVU4-7	$1,099.00
‡SapI linearized vector sufficient for 20 cloning reactions
Plasmid Expression Vector kit, 83 series‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8382	secreted	20 µg	NTC- DV8382	$420.00
NTC8385-EGFP*	cytoplasmic EGFP	20 µg	NTC- DV8385-EGFP	$420.00
NTC8382-VAI	secreted	20 µg	NTC- DV8382VAI	$420.00
NTC83825-VAI-EGFP*	cytoplasmic EGFP	20 µg	NTC- DV8385VAI-EGFP	$420.00
*The NTC8385-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC8385 backbone for cloning new genes ‡Kit contains plasmid and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	XL1-Blue dcm- host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC5	$199.00
Linearized Vector kit 83 series‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8382	secreted	1 µg	NTC- DV8382-LV	$444.00
NTC8385-EGFP	native	1 µg	NTC- DV8385--LV	$444.00
NTC8382-VAI	secreted	1 µg	NTC- DV8382VAI-LV	$444.00
NTC8385-VAI-EGFP	native	1 µg	NTC- DV8385VAI-LV	$444.00
‡SalI - BglII linearized vector sufficient for 20 cloning reactions and DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain glycerol stock
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	NTC48165 DH5α dcm- host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC2	$199.00
NTC54208	XL1-Blue dcm- host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC5	$199.00
NTC54208	NTC5402 XL1-Blue host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC6	$199.00
Plasmid Expression Vectors Kanamycin Selection
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC7482	secreted	20 µg	NTC- DV7482	$320.00
NTC7485-EGFP*	cytoplasmic EGFP	20 µg	NTC- DV7485-EGFP	$320.00
*The NTC7485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC7485 backbone for cloning new genes
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54207	XL1-Blue dcm- host strain for 7-series vectors selection	0.5 ml	NTC-DVU-CC4	$199.00
RIG-1-activating DNA Vaccine Plasmids and Linear Cloning Vectors: Kanamycin Selection Plasmid Expression Vectors
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC7482-eRNA41H	secreted	20 µg	NTC- DV7482-41H	$320.00
NTC7485-eRNA41H-EGFP*	cytoplasmic EGFP	20 µg	NTC- DV7485-41H-EGFP	$320.00
*The NTC7485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC7485 backbone for cloning new genes
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	NTC48107 DH5α dcm- host strain for 7-series vectors	0.5 ml	NTC-DVU-CC3	$199.00
NTC54207	XL1-Blue dcm- host strain for 7-series vectors selection	0.5 ml	NTC-DVU-CC4	$199.00
Linearized Vectors‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC7482	secreted	1 µg	NTC- DV7482-LV	$344.00
NTC7485	native	1 µg	NTC- DV7485-LV	$344.00
‡SalI - BglII linearized vector sufficient for 20 cloning reactions
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54207	XL1-Blue dcm- host strain for 7-series vectors selection	0.5 ml	NTC-DVU-CC4	$199.00
NTC offers the following accessory products for use cloning with the NTC7482 and NTC7485 vectors.
XAccessory Products	XDescription	XQuantity	XCatalogue no.	PriceX
pVAX5’	Forward sequencing primer	500 pmol (10 pmol/µL)	NTC-DVU-SP1	$30.00
pVAX3’	Reverse sequencing primer	500 pmol (10 pmol/µL)	NTC-DVU-SP2	$30.00
Plasmid Expression Vector kit‡ Antibiotic-Free (RNA-OUT) Vectors
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8482	secreted	20 µg	NTC- DV8482	$420.00
NTC8485-EGFP*	cytoplasmic EGFP	20 µg	NTC- DV8485-EGFP	$420.00
* The NTC8485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC8485 backbone for cloning new genes ‡Kit contains plasmid and DH5α att_λ::P_{5/6 6/6}-RNA-IN- SacB, catR host strain glycerol stock
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	XL1-Blue dcm- host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC5	$199.00
Linearized Vector kit‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8482	secreted	1 µg	NTC- DV8482-LV	$444.00
NTC8485	native	1 µg	NTC- DV8485-LV	$444.00
‡SalI - BglII linearized vector sufficient for 20 cloning reactions and DH5α att_λ::P_{5/6 6/6}-RNA-IN- SacB, catR host strain glycerol stock
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	XL1-Blue dcm- host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC5	$199.00
NTC offers the following accessory products for use the NTC8482 and NTC8485 vectors
XAccessory Products	XDescription	XQuantity	XCatalogue no.	PriceX
pVAX5’	Forward sequencing primer	500 pmol (10 pmol/µL)	NTC-DVU-SP1	$30.00
pVAX3’	Reverse sequencing primer	500 pmol (10 pmol/µL)	NTC-DVU-SP2	$30.00
NTC4862 DH5α att_λ::P_{5/6 6/6}-RNA-IN-SacB, catR electrocompetent cells	Host strain for NTC8482 and NTC8485 selection	0.5 mL	NTC-DVU-CC1	$199.00
Sucrose selection agar	Plates for NTC8482 and NTC8485 selection	Pouch to make 1 L agar*	NTC-DVU-MD1	$20.00
Sucrose selection media	Media for NTC8482 and NTC8485 culture	Pouch to make 1 L media*	NTC-DVU-MD2	$20.00
*Reconstitute with distilled water to 1L and autoclave prior to use
Sucrose Selection Plasmid Expression Vector kit‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8482-eRNA41H	secreted	20 µg	NTC- DV8482-41H	$420.00
NTC8485-eRNA41H-EGFP*	cytoplasmic EGFP	20 µg	NTC- DV8485-41H-EGFP	$420.00
* The NTC8485-EGFP control plasmid is used as a transfection control for expression in a cell line of interest, and as the NTC8485 backbone for cloning new genes ‡Kit contains plasmid and DH5α att_λ::P_{5/6 6/6}-RNA-IN- SacB, catR host strain glycerol stock
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	XL1-Blue dcm- host strain for 8-series vector selection	0.5 ml	NTC-DVU-CC5	$199.00
Sucrose Selection Linearized Vector kit‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8482 eRNA41H	secreted	1 µg	NTC- DV8482-41HLV	$444.00
NTC8485 eRNA41H	native	1 µg	NTC- DV8485-41HLV	$444.00
‡SalI - BglII linearized vector sufficient for 20 cloning reactions and DH5α att_λ::P_{5/6 6/6}-RNA-IN- SacB, catR host strain glycerol stock
XHost cell line	XDescription	XQuantity	XCatalogue no.	PriceX
NTC54208	XL1-Blue dcm- host strain for 8-series vectors selection	0.5 ml	NTC-DVU-CC5	$199.00
Recombineering Vectors
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
pKD46-recA	Escherichia coli	20 µg	NTC-RV-RecA	$320.00
pKD46-recApa	Pseudomonas aeruginosa	20 µg	NTC-RV-RecApa	$320.00
NTC86 Series Expression Vectors, Antibiotic-Free selection‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8681 vector kit	endosome	20 µg	NTC-DV8681	$420.00
NTC8682 vector kit	secreted	20 µg	NTC-DV8682	$420.00
NTC8684 vector kit	ubiquitin	20 µg	NTC-DV8684	$420.00
NTC8685-EGFP vector kit	cytoplasmic, EGFP	20 µg	NTC-DV8685-EGFP	$420.00
‡ plus NTC4862 (DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain) glycerol stock
NTC86 Series Targeting/Rig1 Activating Vectors‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC8681-eRNA41H vector kit	endosome	20 µg	NTC-DV8681eRNA41H	$420.00
NTC8682-eRNA41H vector kit	secreted	20 µg	NTC-DV8682eRNA41H	$420.00
NTC8684-eRNA41H vector kit	ubiquitin	20 µg	NTC-DV8684eRNA41H	$420.00
NTC8685-eRNA41H-EGFP vector kit	cytoplasmic, EGFP	20 µg	NTC-DV8685-eRNA41H-EGFP	$420.00
‡ plus NTC4862 (DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain) glycerol stock
NTC8682/NTC8685 Linear Vectors‡
XVector	XTargeting	XQuantity	XCatalogue no.	PriceX
NTC-8682-LV	secreted	1 µg	NTC-DV8682-LV	$444.00
NTC-8685-LV	native	1 µg	NTC-DV8685-LV	$444.00
‡ plus NTC4862 (DH5α attλ::P5/6 6/6-RNA-IN- SacB, catR host strain) glycerol stock
NTC Precision Contract Cloning Services:
XAn economical, rapid service whereby NTC clones investigator-specified genes into the desired expression Xvectors, providing sequence-validated clones, or purified plasmid DNA ready for animal testing:
XDescription		XCatalogue no.		Price
Seamless cloning gene of interest into any vector		NTC-RVC1		$400.00
1mg endotoxin-free plasmid preparations, each		NTC-RP1		$335.00
Vector resistance retrofit service, <7kb, PCR cloning		NTC-VRFRES-S		$500.00
Vector resistance retrofit service, >7kb, in vivo		NTC-VRFRES-L		$750.00

*Autolytic E. coli* Cell Lines Pricing**
Strain	Genotype	Induction	Catalog Number	Price
NTC3012	DH5α att_HK022::P_R-zwf- λR, strR	37-42°C	NTC-DH5-AL_HEAT(Glycerol stock)	$100.00
NTC3012 competent cells	DH5α att_HK022::P_R-zwf- λR, strR	37-42°C	NTC-DH5-AL_HEAT-CC1 (10x100µL competent cells)	$199.00
NTC40116	DH5α att_HK022::P_tac-zwf- λR, strR	IPTG	NTC-DH5-AL_IPTG(Glycerol stock)	$100.00
NTC40116 competent cells	DH5α att_HK022::P_tac-zwf- λR, strR	IPTG	NTC-DH5-AL_IPTG-CC1 (10x100µL competent cells)	$199.00
NTC4828	BL21 att_HK022::P_R-zwf- λR, strR	37-42°C	NTC-BL21-AL_HEAT(Glycerol stock)	$100.00
NTC4828 competent cells	BL21 att_HK022::P_R-zwf- λR, strR	37-42°C	NTC-BL21-AL_HEAT-CC1 (10x100µL competent cells)	$199.00
NTC40117	DH5α att_HK022::P_tac-zwf- λR, strR	IPTG	NTC- BL21-AL_IPTG(Glycerol stock)	$100.00
NTC40117 competent cells	DH5α att_HK022::P_tac-zwf- λR, strR	IPTG	NTC- BL21-AL_IPTG-CC1 (10x100µL competent cells)	$199.00
DH5α: F- Φ 80lacZΔM15 Δ(lacZYA -argF) U169 recA1 endA1 hsdR17(r_K^-, m_K⁺) gal phoA supE44 λ- thi-1 gyrA96 relA1 BL21: F- ompT hsdSB (r_B- m_B-) gal dcm

dmc-E. coli Cell Lines Pricing
Strain	Catalog Number	Price
NTC4862 DH5a host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC1	$199.00
NTC48165 DH5a dcm- host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC2	$199.00
NTC48107 DH5a dcm-NTC48107 DH5a dcm- host strain for 7-series vectors, 0.5 mL	NTC-DVU-CC3	$199.00
NTC54207 XL1-Blue dcm- host strain for 7-series vectors, 0.5 mL	NTC-DVU-CC4	$199.00
NTC54208 XL1-Blue dcm- host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC5	$199.00
NTC5402 XL1-Blue host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC6	$199.00

shRNA Expression Vectors (RNA Interference)	Catalogue No.	Retail Price
NTC7485E-U6-shRNA vector, KanR, 20 µg	NTC-RI7485E-U6	$299.00
NTC8485E-U6-shRNA vector, antibiotic free, 20 µg	NTC-RI8485E-U6	$299.00
NTC8485-U6-shRNA vector, antibiotic free, 20 µg	NTC-RI8485-U6	$299.00
Vector, KanR, 20µg	NTC-RIPMLV-U6	$299.00
pMLV GFP-neo H1 vector, KanR, 20 µg	NTC-RIPMLV-H1	$299.00
NTC7485E-U6-shRNA linearized vector, gel isolated, KanR, 1 µg	NTC-RI7485E-U6LV	$344.00
NTC8485E-U6-shRNA linearized vector, gel isolated, antibiotic free, 1 µg	NTC-RI8485E-U6LV	$344.00
NTC8485-U6-shRNA linearized vector, gel isolated, antibiotic free, 1 µg	NTC-RI8485-U6LV	$344.00
pMLV GFP-neo U6 linearized vector, gel isolated, KanR, 1 µg	NTC-RIPMLV-U6LV	$344.00
pMLV GFP-neo H1 linearized vector, gel isolated, KanR, 1 µg	NTC-RIPMLV-H1LV	$344.00
pRIP U6 5' forward sequencing primer, 500 pmol (10 pmol/μL)	NTC-DVU-RI1	$30.00
pRIP H1 5' forward sequencing primer, 500 pmol (10 pmol/μL)	NTC-DVU-RI2	$30.00
shRNA cloning service; customer-defined shRNA into vector and sequence verification	NTC-RI-CLON	$400.00
Vector dimerization service	NTC-VDIM	$400.00
Vector promoter retrofit service	NTC-VRFPRO	$500.00
NTC4862 DH5 alpha host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC1	$199.00
NTC48165 DH5 alpha dcm- host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC2	$199.00
NTC48107 DH5 alpha dcm- host strain for 7-series vectors, 0.5 mL	NTC-DVU-CC3	$199.00
NTC54207 XL1-Blue dcm- host strain for 7-series vectors, 0.5 mL	NTC-DVU-CC4	$199.00
NTC54208 XL1-Blue dcm- host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC5	$199.00
NTC5402 XL1-Blue host strain for 8-series vectors selection, 0.5 mL	NTC-DVU-CC6	$199.00
Sucrose selection agar for 8-series vectors selection, makes 1L agar	NTC-DVU-MD1	$20.00
Sucrose selection media for 8-series vectors culture, makes 1L media	NTC-DVU-MD2	$20.00

ABOUT NTC PUBLICATIONS PATENTS RESEARCH AND DEVELOPMENT IP LICENSING PLATFORMS PARTNERING OPPORTUNITIES

VECTORS | CELL LINES | VACCINES & ADJUVANTS | CLONING | PROTEINS | PLASMIDS | PROCESS DEVELOPMENT | DNA FERMENTATION PROCESS | HYPERTAQ | CONTRACT QC

Vectors 2010 NTC Vaccine/Vector Pricing

VectorsX|XServicesX|XPricingX|XManualsX|XPay Invoices Online

ABOUT NTC
PUBLICATIONS
PATENTS
RESEARCH AND DEVELOPMENT
IP LICENSING PLATFORMS
PARTNERING OPPORTUNITIES

Vectors
2010 NTC Vaccine/Vector Pricing