NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

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N TC'S PUBLICATIONS
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VECTORS | CELL LINES | VACCINES & ADJUVANTS | CLONING | PROTEINS | PLASMIDS | PROCESS DEVELOPMENT | DNA FERMENTATION PROCESS | HYPERTAQ | CONTRACT QC

Plasmid Detox using pINT PL PR Shuttle Vector
NTC strain engineering service for improving yields of toxic plasmids

NTC Contacts:

Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

Vector Selection FlowchartX|XManualsX|XPlasmid Detox using pINT PL PR Shuttle Vector (PDF)

pINT PL PR Shuttle vector Plasmid yields in fermentation culture typically range up to 2g/L, or more, for plasmids with non-toxic inserts. However, some inserts are toxic to E. coli, making it difficult to grow the quantities needed, also resulting in plasmid loss, mutations and deletions. NTC has devised a novel and economical strain engineering service, using the pINT PL PR Shuttle, wherein the host strain chromosome of E. coli is modified to include a gene that is expressed as antisense to the plasmid-encoded toxic gene (1). Elimination of the toxic gene products allows the plasmid to be grown to the high levels needed for producing larger quantities of plasmid.
Easy ~	NTC’s pINT PL PR shuttle makes it fast and simple to knock down expression of toxic genes in E. coli during plasmid DNA production
Economical ~	$1,249 includes: vector; cloning; strain engineering; and testing
Effective ~	Typical four-fold improvement in plasmid yield

Luke, J., Carnes, A.E., Hodgson, C.P., and Williams, J.A. (2011) Vector insert-targeted integrative antisense expression system for plasmid stabilization. Mol Biotechnol. 47:43-49.

XXXXNTC’s Detox Plasmid Yield Improvement Process

ABOUT NTC NTC'S PUBLICATIONS RESEARCH AND DEVELOPMENT IP LICENSING PLATFORMS PARTNERING OPPORTUNITIES

VECTORS | CELL LINES | VACCINES & ADJUVANTS | CLONING | PROTEINS | PLASMIDS | PROCESS DEVELOPMENT | DNA FERMENTATION PROCESS | HYPERTAQ | CONTRACT QC

pINT PL PR Shuttle vector Plasmid yields in fermentation culture typically range up to 2g/L, or more, for plasmids with non-toxic inserts. However, some inserts are toxic to E. coli, making it difficult to grow the quantities needed, also resulting in plasmid loss, mutations and deletions.

ABOUT NTC
N TC'S PUBLICATIONS
RESEARCH AND DEVELOPMENT
IP LICENSING PLATFORMS
PARTNERING OPPORTUNITIES

pINT PL PR Shuttle vector

Plasmid yields in fermentation culture typically range up to 2g/L, or more, for plasmids with non-toxic inserts. However, some inserts are toxic to E. coli, making it difficult to grow the quantities needed, also resulting in plasmid loss, mutations and deletions.