NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

NTC Precision Cloning
Vaccine Vectors – FAST!


NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing
Precision Cloning is a streamlined process whereby NTC provides you with custom-finished DNA vaccine vectors, sequence-validated and ready for animal testing, in record time - a new weapon in the arsenal against infectious diseases, bioterrorism and cancer therapy.

Vector Design:
NTC scientists work with clients for design optimization

Insert Choices:
• Client Genes
• External Sources
• Optimized Synthetic Genes


Vector Choices:
• Optimized NTC DNA Vaccine Vectors
• Optimized NTC Therapeutic Gene Vectors


Technology Advantages:
• Vectors optimized for: speed (4-5 weeks typical turnaround times); economy ($400 typical cost per antigen, includes sequencing inserts), regulatory compliance (Points to Consider); optimal prokaryotic plasmid yield; optimal mammalian transient expression; choice of markerless (antibiotic-free) or KanR; immunogenicity.


• Technology Options: targeting, dcm methyla-ion (+/-), RIG-1 activation (interferon)


Clone Validation:
Fully sequenced inserts and junctions

Plasmid DNA Manufacturing:
Highly purified, endotoxin-free, research grade pDNA

• Small Scale: 1-99mg
• Large Scale: 100mg-Kg
• (HyperGRO™) Fermentation, highest attainable yields
xand purity)

GMP Manufacturing:
Technology Transfer from NTC to CROs, and/or client facilities

Research review paper
Plasmid DNA vaccine vector design: Impact on efficacy,
safety and upstream production
James A. Williams, Aaron E. Carnes, Clague P. Hodgson
XXNTC Precision Cloning Services

An economical, rapid service whereby NTC clones investigator-specified genes into the desired expression vectors, providing sequence-validated clones, or purified plasmid DNA ready for animal testing.
XXPricing

Description
Catalogue no.
Price
Seamless cloning gene of interest into any vector
NTC-RVC1
$400.00
1mg endotoxin-free plasmid preparations, each
NTC-RP1
$335.00
Vector resistance retrofit service, <7kb, PCR cloning
NTC-VRFRES-S
$500.00
Vector resistance retrofit service, >7kb, in vivo
NTC-VRFRES-L
$750.00
Codon Optimization Design Service                                                  
NTC-CONS-CODON
$400.00†
Codon Optimization Report (sample C of A)
No Charge

† No charge with 100 mg endotoxin-free plasmid preparation