Anti-Silencing Elements (ASEs)
For prolonged expression of integrated genes in mammalian cells.

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing


ASEs Advantages

• Maximal and prolonged expression after genome integration

• Decreased gene silencing during cell line passage

• Minimal prokaryotic backbone with antibiotic-free (AF) selectable marker

 

Gene silencing is a common phenomenon that is encountered when genes or vectors are integrated into the recombinant host cell chromatin.  NTC now introduces ASE elements to permit maximum gene expression long after integration (Fig. 1).
XXNTC’s ASE vector prep services:


• Swap your gene into the vector (replaces EGFP)*

• Produce one milligram of plasmid DNA

• Linearize 100 microgram (BspHI or XmnI)

• Linearized vector, and remaining uncut vector is shipped to you for integration into your cell lines

*Integrated cell lines containing the neo gene will be selectable with geneticin (other selectable marker swaps available).

 
XXASE Expression


ASE Expression