Linearized Vectors
Custom linearized vectors for high-throughput genomics and screening are created at NTC, effectively solving the vexing problems of digestion, gel isolation, and background (such as residual DNA that is refractile to digestion, stray insert fragments, agarose inhibitors, etc.) The answer is clear: use NTC’s vector production service and full validation profiling: Typically double-digested, gel-isolated DNA, appearing as a single band on an anagarose gel – or custom prepared to your specifications, with background reduced from 104 to105 fold.
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The initial and limiting factor in a library or high-throughput cloning project is the vector. Careful preparation of the vector using a proprietory process gives the highest cloning efficiency, and the lowest background (‘blues’ and empty clones).
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Unlike over-digestion, which does not rid the preparation of unwanted, intact vector. NTC's process results in clean, low-background linearized vectors that you can use with confidence, knowing that the product wil give you the highest cloning efficiency possible.
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