|
Ingredients
Amines bottle – powdered premix
Carbon Source – 460 ml
Trace minerals – 40 ml
Vitamins – 1 ml
|
A representative bioreactor using HyperGRO SB with E. coli strain DH5α and a typical high-copy (pUC or comparable) backbone, results in >200mg/L of plasmid culture, approximately 50 fold higher than equivalent shake flask volumes.
|
Protocol
1. Dissolve contents of amines bottle in 3500 ml of distilled or deionized water.
2. Add the amines solution to the fermentor vessel and prepare the vessel for auto claving.
3. Insert the vessel into an autoclave and sterilize.
4. After autoclaving, allow the vessel contents to reach operating temperature.
5. Aseptically add entire contents of the carbon source bottle, trace minerals bottle, and vitamins vial. An appropriate antibiotic may also be added at this time. The fermentation medium is now ready for use. A representative bioreactor using HyperGRO SB with E. coli strain DH5α and a typical high-copy (pUC or comparable) backbone, results in >200mg/L of plasmid culture, approximately 50 fold higher than equivalent shake flask volumes.
Notes: 28-30% Ammonium hydroxide and 10% phosphoric acid are recommended for pH control. If the use of antifoam is desired, an initial amount of 0.5 – 2.0 ml may be added to the vessel before autoclaving.
Hardware requirements: Compatible with benchtop bioreactors, pilot plants, and large scale fermentation equipment. User provides: antibiotic, ph control, oxygen and anti-foam, if necessary. Commercial and non-comercial site licenses available.
Please request skid pricing and quantity discounts.
Patents Pending: US and International
|
Hardware requirements: Compatible with benchtop bioreactors, pilot plants, and large scale fermentation equipment. User provides: antibiotic, ph control, oxygen and anti-foam, if necessary. Commercial and non-comercial site licenses available.
|
|
