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DNA Vaccines: Rapid Development and Rapid Deployment
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DNA vaccines offer a new opportunity to immunize with materials that are entirely gene-based, expressed by the recipient’s own cells. This means that there is greater control over the immunization process, because the investigator determines which antigens and co-stimulants to use, where to elicit the response (e.g., skin or muscle), and which cytokines or innate controls (if any) to use. The duration of the response can be controlled by repeated exposure to the genes, which are expressed transiently, by a variety of delivery mechanisms such as: direct injection; electroporation; gene gun; mucosal delivery, etc. The ability to make DNA molecules strictly by rational design makes it possible to bypass months of development for the production of efficacious vaccines. DNA vaccines are expressed and presented in the host, making an ideal mimic of intracellular antigens. Thanks to several emerging technologies, NTC offers complete consulting and vector development services for DNA vaccines, including the full range of design, construction and manufacturing services. NTC's GENSA™(Gene Self-Assembly)(PDF) technology is a key part in the vaccine development process, saving sub-cloning steps by recombining all genes and DNA fragments at once. NTC can do the work for you in record time to save facility and staffing concerns.
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XXXVersatile Targeting DNA Vaccine Vectors
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Versatile targeting DNA vaccine vectors are designed to maximize versatility and flexibility combining minimal prokaryotic sequences, kanamycin selectable marker, and highest level expression with the ability to target individual compartments (secreted, endosomal, membrane-anchored, intracellular-processing targeted, or native). The vectors are easily modified to seamlessly substitute any or all gene modules.
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| XXXpDNAVACC-Ultra, Vectors designed to maximize versatility and flexibility (PDF) |
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Versatile targeting DNA vaccine vectors are designed to maximize versatility and flexibility combining minimal prokaryotic sequences, kanamycin selectable marker, and highest level expression with the ability to target individual compartments (secreted, endosomal, membrane-anchored, intracellular-processing targeted, or native). The vectors are easily modified to seamlessly substitute any or all gene modules.
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•Versatile because it combines minimal prokaryotic sequences, kanamycin selectable marker, and highest-level expression with the ability to target individual compartments (secreted, endosomal, membrane-anchored, intracellular-processing targeted, or native).
•Flexible because it can be easily modified to seamlessly substitute any or all gene modules using GENSA™ (Gene Self-assembly)*.
*The modular GENSA approach allows operator control over the promoters, enhancers, expressed genes, leaders, introns, polyadenylation signals, selectable markers, site-directed mutagenesis, etc. The seamlessness of the GENSA approach allows vectors to be modified at any base (through insertion, deletion or mutation), using unique address tags and a proprietary cloning process that allows the plasmids to be modified ad libitum.
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XXXRapidVACC™ Vaccine Vectors – FAST!
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Rapid-VACC™ is streamlined process whereby we provide you with custom-finished DNA vaccine vectors, sequence-validated and ready for animal testing, in record time - a new weapon in the arsenal against infectious diseases, bioterrorism and cancer therapy. Call for more information.
• Rapid assembly of antigens and adjuvant genes into pDNAVaccUltra vectors
• Rapid screening of candidate vaccine vectors using Multi-Mode presentation
• Rapid optimization and improvement
• Rapid development of protein boost with pVex antigen expression system
High throughput DNA vaccine plasmid generation is a quick, cost effective method for generating intracellular targeted DNA vaccines. In today's world, emerging infectious diseases (like SARS, anthrax, smallpox, West Nile, monkeypox and bird flu ) and bioterrorist threats can appear on our doorstep and spread like wildfire. Now it is possible to create technological weapons against these threats in weeks, instead of months or years.
RapidVACC™ high throughput DNA vaccine plasmid generation is a quick, cost effective process for targeted DNA vaccine vectors. We provide four plasmids, each with different destinations, for your gene of interest. Each plasmid utilizes NTC’s proprietary pDNA-VACC-Ultra™ plasmid backbone, optimized for both high level expression of target antigens and for harmonization with FDA points to consider on plasmid DNA vaccines. Immunization with the series of plasmids allows you to rapidly determine the optimal MHC presentation of your protein.
RapidVacc Specifications:
NTC will PCR amplify and clone your gene into NTC’s DNA vaccine expression vector A series using your template and sequence data, sequence insert and ship 4 cell lines to you for in as little as three weeks!
You will receive cell lines* containing your gene in NTC' s pDNAVACC-ultra.
A1: secreted
A2: membrane anchored
A3: intracellular processing targeted
A4: native
Call us for a quote for larger or smaller amounts, or high-throughput pricing.
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Contact NTC, Toll free: 1-(888) WIz-Bang
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