DNA Fermentation Process
NTC's, HyperGRO™ Plasmid DNA (pDNA) Fermentation Technology

HyperGRO™, DNA Fermentation ProcessX|XLicensingX|XHyperGRO Prices X|XInstructions X|XE. coli Cell Paste

• 1 - 1.5 g/liter plasmid DNA production • Fermentation process for batch and fed-batchmodes of production • Animal Product Free (APF) guarantee • Scalable custom fermentation

The benefits of the HyperGRO™ fermentation process are well known: the best specific and volumetric yields attainable (1.5-2g/L); highest purity; and greatest economy. results from many years of research aimed at maximizing plasmid DNA yield, while maintaining the fidelity and integrity of the plasmid.  Developed by Nature Technology Corporation, a leader in bioproduct and bioprocess development, HyperGRO is an optimized, semi-defined media process that allows researchers to increase their typical plasmid yield dramatically over other methods. Custom batched kits are the way to go with occasional use, while site licensing (commercial, non-comercial) is available for regular users, such as CMOs and GMP production facilities.

XXXReady to use for maximum E. coli fermentation yield of plasmid DNA

Fermentation ASGT 2006: Poster 2006

Batch Process Kits -------------------------------------- • Typical yields >200mg/L • Easy to use • Custom made for your fermenter • All veggie, APF materials • Well-characterized, technical grade and research grade

Fed Batch Process Kits -------------------------------------- • Typical yields, up to 1.5 gram/liter • Easy to use • Custom made to order • All veggie, APF materials • Well-characterized, technical grade and research grade

The unique components of the HyperGRO process contribute significantly to the plasmid production requirements of E. coli.  HyperGRO consistently outperforms published methods, and is highly compatible with plasmid purification processes.   The HyperGRO process a ready-to-use product, composed of a proprietary blend of amines, trace minerals, vitamins and carbon sources optimized for maximal plasmid specific yield. Yield may vary based on the plasmid/host combination used. Observed yields vary from approximately 100-250mg/l (SB) or 150-2,000mg/l (SFB) with average plasmids having pUC- or PBR-derived origins of replication, grown in E. coli host DH5a. For maximizing yields, we recomend the combination of an optimizing pUC plasmid backbone (such as NTC7264) and DH5a.

Figure 1. Typical yields for shake flask cultures vs. HyperGRO batch and fed batch or fed batch modes of fermentation.

Fed-batch HyperGRO™ (Patents Pending) fermentation using NTC7264 backbone with 30-42C temperature shift, yield 1,500mg/L.

Do it yourself with the HyperGRO™ process or let us provide you with the highly concentrated,
E. coli Cell Paste containing your plasmid DNA.

Contact NTC, Toll free: 1-(888) WIz-Bang