Plasmid DNA Purification and Processing (1-99mg)
Small Scale Plasmid DNA Purification

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing
X

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NTC is an industry leading provider of highly-purified plasmids. Ordering highest quality plasmid DNA from NTC is a fast, easy and economical process.

Timeline: First time orders require 2-3 weeks. Reorders can be from 1 day to two weeks, depending upon the arrangements made (just in time re-ordering is by prior agreement).

Plasmid Order Form | C of A | Additional Testing
XXEasy to Order:

1) Call NTC for a quotation. Best pricing is available based on quantity ordered and copy number. Most high-copy plasmids have a pUC origin of replication, or equivalent, and result in the lowest cost. NTC is a longtime industry leader in quality and cost-effectiveness.

2) Send NTC 1 microgram of your plasmid DNA, spotted on Whatman 3MM or FTE filter paper, together with NTC's Plasmid DNA Order Form (The form is designed to prompt you for all information needed for propagation and diagnostic testing.)
XXNTC will:

• Transform the production host.

• Prepare an archival glycerol stock using "low metabolic burden technology" ,

• Grow, and purify the desired quantity of pDNA, using endotoxin-free reagents and procedures. 

• Formulate the DNA according to specification (default is 0.5mg/ml in TE buffer). 

• A small-scale certificate of analysis will be provided, including: gel photo (digested and undigested DNA, ladder), concentration and quantity:

Small Scale Certificate of Analysis (C of A)

XXXXX·  Spectrophotometric analysis (A260/280)
XXXXX·  Quantity
XXXXX·  Concentration (usually 0.5mg/ml, in TE buffer)
XXXXX·  Volume
XXXXX·  Gel photo (digested and undigested DNA, ladder)
XXXXX·  Qualitative analysis (pass/fail)


In addition, NTC will retain an archival lot sample, and a glycerol stock of your plasmid for future reorders (at least one year retention).
XXNTC's testing at no additional charge and cost comparison with NTC's competitors



XXAdditional testing is available upon request
Additional testing

Residual Protein test (modified BCA)--small-scale pDNA prep
NTC-RPSS-RPA
$75.00

Residual RNA test (spiked gel)--small-scale pDNA prep
NTC-RPSS-RRNA
$75.00

Genomic DNA test (RT-PCR)--small-scale pDNA prep
NTC-RPSS-GDNA
$150.00

Endotoxin test--small-scale pDNA prep
NTC-RPSS-ENDO
$50.00
0.2 um filtration of purified plasmid
NTC-RPSS-FILTR
$50.00

Bioburden test--small-scale pDNA prep
NTC-RPSS-BBDN
$50.00
Residual Protein test (modified BCA)--large-scale pDNA prep NTC-RPLS-RPA
$75.00
Residual RNA test (spiked gel)--large-scale pDNA prep NTC-RPLS-RRNA
$0.00
Genomic DNA test (RT-PCR)--large-scale pDNA prep NTC-RPLS-GDNA
$0.00
Endotoxin test--large-scale pDNA prep NTC-RPLS-ENDO
$0.00
Bioburden test--large-scale pDNA prep NTC-RPLS-BBDN
$50.00

XXRelated molecular services: