NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals
DNA Fermentation Process
HyperGRO™ Fermentation: Plasmid DNA Production Options, 2.6 mg/l

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing


NTC’s HyperGRO™ plasmid DNA fermentation process is today recognized as the industry Gold Standard for making
high-yield, low-cost, high-purity DNA vaccines and gene therapeutics (Fig. 1, Refs.1-5).

Comparative yields, DNA fermentation

Fig. 1.

A) Yield comparison, typical vs. HyperGRO™.

B) HyperGRO™ fermentation process schematic.

C) Fed-batch HyperGRO™ fermentation using NTC7264 backbone with 30-42C temperature shift, yield 1,500mg/L (left scle, mg/L).

XXNTC customers have several options:

1) Complete Plasmid DNA Purification Services: Small scale (1-99mg), and Large scale (100mg-kg) custom-purified, research-grade DNA is available directly from NTC. Orders 500mg and up are generally made using HyperGRO™. In addition, GMP grade DNA is available through NTC’s GMP manufacturing partners (inquire).



2) Process Development/Cell Paste: In order to see if your plasmid is compatible with the HyperGRO™ fed-batch fermentation process, shake-down runs are available, in which an NTC engineer will optimize fed-batch fermentation for your host cell/plasmid combinations. We will concentrate, freeze and provide you with the cell paste (Fig. 2) from optimal runs if you would like to purify it elsewhere, or NTC can complete the purification.



3) Technology Transfer to your facility: An NTC engineer will set up the process in your facility and train technical staff to make the world’s best DNA. NTC will provide all technical documentation.
XXHyperGRO™ Publications

Plasmid DNA Fermentation Strain and Process-Specific Effects on Vector Yield, Quality and Transgene Expression
Carnes, A.E., Luke, J.M., Vincent, J.M., Schukar, A., Anderson, S., Hodgson, C.P., Williams, J.A. Biotechnol. Bioeng. (2011). Feb;108(2):354-63.


Plasmid DNA production combining antibiotic-free selection, inducible high yield fermentation, and novel autolytic purification,
Carnes, AE, Hodgson, CP, Luke, JM, Vincent, JM, Williams, JA, Biotechnol. Bioeng. 104:505-515 (2009)


Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes,
James A Williams *, Jeremy Luke, Sarah Langtry, Sheryl Anderson, Clague P Hodgson, Aaron E Carnes, Nature Technology Corporation, Lincoln, NE, USA. Biotechnol. Bioeng. (2009) 103:1129-1143


Rapid process development for high yielding plasmid DNA fed-batch fermentation.
Williams, J.A., Hodgson, C.P., and Carnes, A.E. (2009).BioPharm International 22(11):46-51


Inducible Escherichia coli fermentation for increased plasmid DNA production,
Carnes AE, Hodgson CP, Williams, JA. (2006) Biotechnol. Appl. Biochem. 45:155-166.