NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

DNA Fermentation Process
E. coli cell paste


NTC Contacts:

Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292

Email: support@natx.com


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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X
Antibiotic-Free Vectors
XRig-I Activating Vectors
XLinear Vectors
XRecombineering Vectors
XGene Silencing (transient)
XGene Silencing (retroviral, stable)
XAnti-Silencing Elements
XVector Pricing

CELL LINES
XAutolytic E. coli Cell Lines
X
dcm- Strains

VACCINES & ADJUVANTS
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DNA Vaccines
X
Rig-I Activating DNA Vaccines
XThermostable Protein Expression

PRECISION CLONING
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Services and Pricing

RECOMBINANT PROTEINS
X
pVEX™-E. coli Expression Vectors
X
Fluorescent Proteins
X
Products, Vectors, Services, Prices
XCofA (SAMPLE)

PLASMIDS
XSmall Scale Plasmid DNA
XSmall Scale C of A
XLarge Scale Plasmid DNA
XLarge Scale C of A
X
Plasmid DNA Order Form
XE. coli Cell paste
XNEW! Plasmid Detox Service
X
Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
XHyperGRO™ Publications
XE. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE


CONTRACT QC
X
E. coli Genomic DNA Testing
X
Residual RNA qPCR
XResidual RNA gel
X
Supercoil Assay
X
Lymulus Endotoxin Testing


Plasmid Fermentation E. coli Cell Paste, for highest yield plasmid DNA using the HyperGRO process:

$3,200/g,  additional grams, if available, are $1,000/g

E. coli cells after CEPA centrifuge separation


Do you want to purify pDNA with your own downstream process, but you also want to take advantage of NTC’s HyperGRO fed-batch fermentation technology?  If so, you may want to order your plasmid as frozen, concentrated cell paste, directly from NTC.  NTC will perform a 10L fed-batch HyperGRO fermentation using its optimal process (typical yields: 300mg-2.6g/L), and send you the equivalent of up to 1g of pDNA in the form of paste (or, the amount available from 10L), for $3,200.  Additional grams, if available, are $1,000/g.  Additional fermentations are available upon request, and may result in greater yields due to knowledge gleaned from early fermentations.


Process Development Fermentations:


Paste fermentations are thus incorporated into process development, and may be ordered for that purpose alone, or they may be combined with purification in order to obtain pDNA.  If you choose to have NTC purify the DNA, the cost of the fermentation ($3,200) will be deducted from the regular cost of purified DNA. 

XXNTC offers full downstream purification services

Process Other Options:

Large scale plasmid DNA Production

Small Scale Plasmid DNA

HyperGROkit includes: kit license and requires exponential feed capability.


Site licenses:

For contract research facilities or for in-house production.


Biotech Suppliers:

Consider out-sourcing your company's entire plasmid production effort to NTC, saving you lots of money and allowing you to re-prioritize your staff. Your orders will receive creful management from our knowledgeable and experienced professionals. Timely delivery and high quality products are guaranteed with NTC' industrial outsource program. Perfect for biotech suppliers and vector banks.