2011 Publications

Plasmid DNA Fermentation Strain and Process-Specific Effects on Vector Yield, Quality and Transgene Expression
Carnes, A.E., Luke, J.M., Vincent, J.M., Schukar, A., Anderson, S., Hodgson, C.P., Williams, J.A. Biotechnol. Bioeng. (2011). Feb;108(2):354-63.

Vector Insert-Targeted Integrative Antisense Expression System  for Plasmid Stabilization
Luke, J.M., Carnes, A.E., Hodgson, C.P., Williams, J.A. Mol. Biotechnol. (2011). 47(1):43-9.

Coexpressed RIG-I Agonist Enhances Humoral Immune Response to Influenza DNA Vaccine
Luke, J.M., Simon, G.G., Soderholm, J., Errett, J.S., August, J.T., Gale, J., Hodgson, C.P., Williams, J.A. (2011). J. Virol. 85:1370-83.

Thermostable Tag (TST) protein expression system: engineering thermotolerant recombinant proteins and vaccines
Luke, J., Carnes, A.E., Sun, P., Hodgson, C.P., Waugh, D.S., and Williams, J.A., (2011). J. Biotechnol., 151:242-50.


Improved antibiotic-free plasmid vector design by incorporation of transient expression enhancers
Luke, J., Vincent, J.M., Du, S.X., Whalen, B., Leen, A., Hodgson, C.P., and Williams, J.A.. Gene Ther.18:334-343 (2011)

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2010 Publications

Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields
Carnes, A.E., Luke, J.M., Vincent, J.M., Anderson, S., Schukar, A, Hodgson, C.P., and Williams, J.A. J. Gene Medicine, (12(10):818-31).

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2009 Publications

Plasmid DNA production combining antibiotic-free selection, inducible high yield fermentation, and novel autolytic purification, Carnes, AE, Hodgson, CP, Luke, JM, Vincent, JM, Williams, JA, Biotechnol. Bioeng. 104:505-515 (2009)


Strain engineering by genome mass transfer: efficient chromosomal trait transfer method utilizing donor genomic DNA and recipient recombineering hosts, Williams, JA, Luke, J, Hodgson, CP, Mol. Biotechnol. (2009) 43:41-51


Improved antibiotic-free DNA vaccine vectors utilizing a novel RNA based plasmid selection system, Luke J, Carnes AE, Hodgson CP, Williams JA. Vaccine 27(46):6454-9.(2009)


Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes, James A Williams *, Jeremy Luke, Sarah Langtry, Sheryl Anderson, Clague P Hodgson, Aaron E Carnes, Nature Technology Corporation, Lincoln, NE, USA. Biotechnol. Bioeng. (2009) 103:1129-1143


Low metabolic burden plasmid production, Carnes AE, Williams JA, Gen Eng. Biotech News, (2009) 29:56-57


Plasmid DNA vaccine vector design; impact on efficacy, safety and upstream production, Williams, J.A., Carnes, A.E., and Hodgson, C.P. (2009). Biotechnology Advances. (2009) 27:35-370

Rapid process development for high yielding plasmid DNA fed-batch fermentation. Williams, J.A., Hodgson, C.P., and Carnes, A.E. (2009).BioPharm International 22(11):46-51

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2007 Publications


Plasmid DNA manufacturing technology review, Carnes, A., Williams, J., (2007) Current Patents on Biotechnol. 1(2):151-166.C

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2006 Publications


pDNAVACCultra vector family: high throughput intracellular targeting DNA vaccine plasmids, James A Williams, Jeremy Luke, Lance Johnson, Clague Hodgson, (2006) Vaccine 24:4671-4676