NATX Plasmids, for: DNA Vaccines, Gene Therapeutics, Viral Vectors andBiophamacueticals

NTC’s IP licensing platforms
Biologics Development Services: from Concept to Product

NTC Contacts:
Phone: (402) 323-6289 (natx)
Fax: (402) 323-6292
Email: support@natx.com

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NTC PRODUCTS AND SERVICES

MAMMALIAN EXPRESSION VECTORS
X• Antibiotic-Free Vectors
X• Rig-I Activating Vectors
X• Linear Vectors
X• Recombineering Vectors
X• Gene Silencing (transient)
X• Gene Silencing (retroviral, stable)
X• Anti-Silencing Elements
X• Vector Pricing

CELL LINES
X• Autolytic E. coli Cell Lines
X• dcm- Strains

VACCINES & ADJUVANTS
X• DNA Vaccines
X• Rig-I Activating DNA Vaccines
X• Thermostable Protein Expression

PRECISION CLONING
X• Services and Pricing

RECOMBINANT PROTEINS
X• pVEX™-E. coli Expression Vectors
X• Fluorescent Proteins
X• Products, Vectors, Services, Prices
X• CofA (SAMPLE)

PLASMIDS
X• Small Scale Plasmid DNA
X• Small Scale C of A
X• Large Scale Plasmid DNA
X• Large Scale C of A
X• Plasmid DNA Order Form
X• E. coli Cell paste
X• NEW! Plasmid Detox Service
X• Additional Testing

PROCESS DEVELOPMENT

DNA FERMENTATION PROCESS
X• HyperGRO™ Publications
X• E. coli Cell Paste

HYPERTAQ™ DNA POLYMERASE

CONTRACT QC
X• E. coli Genomic DNA Testing
X• Residual RNA qPCR
X• Residual RNA gel
X• Supercoil Assay
X• Lymulus Endotoxin Testing

IP Licensing PlatformsX|XPartnering Opportuities

XXXImproved Plasmid DNA Fermentation:

Fermentation patent: High yield HyperGRO, fed-batch fermentation process, maximum yields >2.5g/l: PROCESS FOR PLASMID DNA FERMENTATION: PCT/US2005/29238

Autolysis: (divisional) relating to: low temperature glycerol stock; and post fermentation hold:  IMPROVED E COLI PLASMID PRODUCTION STRAINS: PCT/US2008/006553

Anti-insert stabilization of toxic plasmid inserts: BACTERIAL STRAINS WITH IMPROVED PLASMID STABILITY: US61/210,073

XXXImproved Vectors:


Improved DNA vaccines: VECTORS AND METHODS FOR GENETIC IMMUNIZATION: PCT/US2008/006554, including: RIG-1; antibiotic free plasmids; chimeric promoter

1. Type 1 interferon induction via MDA5 activation via VARNA or a derivative

2.  Method of improving the immune response via Type 1 interferon by expression of immunostimulatory dsRNA from a DNA vaccine

3. Method of improving the immune response by including in a DNA vaccine vector a mitochondrial R region

4. Method of production of markerless, antibiotic free plasmids using RNA-IN, RNA-OUT antisense technology

5. An improved eukaryotic promoter comprising CMV and SV40 sequences

Improved plasmid Copy number – PAS-BH and SV40: DNA PLASMIDS WITH IMPROVED COPY NUMBER

Improved DNA vaccines – RIG-1 Activation of innate immune responses: VECTORS AND METHODS OF GENETIC IMMUNIZATION: PCT/US2006/02174

Anti-silencing elements (ASE): Allow prolonged expression of genes after transient or integrated expression: EUKARYOTIC EXPRESSION VECTORS RESISTANT TO TRANSGENE SILENCING: Serial No.:  US61/342,273

Bacterial strains with Improved plasmid stability
Gene Silencing Vectors:

1. Plasmids, shUT DOWN™ transient expression vectors

2. siLENTC™, retroviral vectors

XXXImproved Downstream Processing of Plasmids:


Trade secrets: re: downstream processing

Autolysis: improved, non-alkaline lysis-based  plasmid extraction methods and strains: IMPROVED E COLI PLASMID PRODUCTION STRAINS: PCT/US2008/006553

XXXProtein manufacturing platform:


pVEX  E. coli high-yield recombinant protein production vectors and process: high yield (typically 1-20g/L); fusion tags

Thermostable adjuvant and thermostable antigen: maltodextrin binding protein (MBP)/flagellin/antigen promotes recombinant antigen extraction, solubilization, and immune responses: THERMOSTABLE FUSION PROTEINS AND THERMOSTABLE ADJUVANT: Serial No.: US61/199651

Defined media fermentation

Autolysis – protein extraction: IMPROVED E COLI PLASMID PRODUCTION STRAINS: PCT/US2008/006553

XXXPan retro-vector applications:


Therapeutics, in vitro applications, vaccines (includes US Patents):

Method of gene transfer using retrotransposons, US 5,354,674

Mammalian retrotransposons, US 5,879,933

Vectors for gene transfer, US Patent 6,027,722

Method for transferring a DNA sequence to a cell in vitro, US Patent 6,287,863

Self-Assembling genes, vectors and uses thereof, US 6,410,220

Chimeric Viral packaging signal without gag gene sequences, US Patent 6,573,091

....and two foreign patents