Advanced Strains of Microorganisms for Biopharmaceutical and Industrial Production

GMT is a disruptive NTC technology, which facilitates orderly and predictable transfer of desirable traits between strains. The strategy is to directly transfer genomic DNA from a strain with a desired property into a recipient strain that expresses recombineering genes. It is not necessary to amplify or isolate DNA. GMT replaces older technology, such as phage P1 transduction. For example, genomic DNA becomes efficiently integrated into the recipient host genome through homologous recombination, after transfer, for example, by electroporation. In some cases, amplified DNA is transferred directly from cell lysates, dramatically simplifying the task of genome engineering. [Patent Ref: PCT/US08/06548]

1) Cells are made competent for mass transfer 2) Add DNA 3) DNA is transfected into cells 4) DNA associates with nucleoplasm 5) Donor DNA integration and replication 6) Assimilation and septation 7) Cell division 8) Selection to grow out recombinant strains